TY - JOUR AB - Geobacillus thermoglucosidasius is agram-positive thermophile of industrial interest thatexhibits rapid growth and can utilize a variety ofplant-derived feedstocks. It is an attractive chassisorganism for high temperature biotechnology andsynthetic biology applications but is currently limitedby a lack of available genetic tools. Here we describea set of modular shuttle vectors, including apromoter library and reporter proteins. The compactplasmids are composed of interchangeable modulesfor molecular cloning in Escherichia coli and stablepropagation in G. thermoglucosidasius and otherGeobacillus species. Modules include two origins ofreplication, two selectable markers and three reporterproteins for characterization of gene expression.For fine-tuning heterologous expression from theseplasmids, we include a characterized promoter libraryand test ribosome binding site design. Together,these gene expression tools and a standardizedplasmid set can facilitate modularity and part exchangeto make Geobacillus a thermophile chassis forsynthetic biology. AU - Reeve,B AU - Martinez,Klimova E AU - de,Jonghe J AU - Leak,DJ AU - Ellis,T DO - 10.1021/acssynbio.5b00298 EP - 1347 PY - 2016/// SN - 2161-5063 SP - 1342 TI - The Geobacillus plasmid set: a modular toolkit for thermophile engineering T2 - ACS Synthetic Biology UR - http://dx.doi.org/10.1021/acssynbio.5b00298 UR - http://hdl.handle.net/10044/1/33966 VL - 5 ER -