TY - JOUR AB - We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays. AU - Rizzo,R AU - Alvaro,M AU - Danz,N AU - Napione,L AU - Descrovi,E AU - Schmieder,S AU - Sinibaldi,A AU - Chandrawati,R AU - Rana,S AU - Munzert,P AU - Schubert,T AU - Maillart,E AU - Anopchenko,A AU - Rivolo,P AU - Mascioletti,A AU - Sonntag,F AU - Stevens,MM AU - Bussolino,F AU - Michelotti,F DO - 10.1016/j.snb.2017.09.018 EP - 2150 PY - 2017/// SN - 0925-4005 SP - 2143 TI - Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices T2 - Sensors and Actuators B: Chemical UR - http://dx.doi.org/10.1016/j.snb.2017.09.018 UR - http://hdl.handle.net/10044/1/57168 VL - 255 ER -