TY - JOUR AB - Imprinted genes are regulated according to parental origin and can influence embryonic growth and metabolism and confer disease susceptibility.Here we designed sensitive allele-specific reporters to non-invasively monitor imprinted Cdkn1cexpression in mice and showed that expression was modulated by environmental factors encounteredin utero.Acute exposure to chromatin modifyingdrugs resulted in de-repression of paternally inherited (silent) Cdkn1calleles in embryos that was temporary and resolved after birth.In contrast, deprivation of maternal dietary proteinin uteroprovoked permanent de-repression of imprinted Cdkn1cexpression that was sustained into adulthood and occurred through a folate-dependent mechanism of DNA methylation loss.Given the function of imprinted genes in regulating behavior and metabolic processes in adults, these results establish imprinting deregulation as a credible mechanism linking early life adversity to later-life outcomes.Furthermore,Cdkn1c-luciferasemice offer non-invasivetools to identify factors that disrupt epigenetic processes and strategies to limit their long-term impact. AU - Van,de Pette M AU - Abbas,A AU - Feytout,A AU - McNamara,G AU - Bruno,L AU - To,WK AU - Dimond,A AU - Sardini,A AU - Webster,Z AU - McGinty,J AU - Paul,EJ AU - Ungless,MA AU - French,PMW AU - Withers,DJ AU - Uren,A AU - Ferguson-Smith,AC AU - Merkenschlager,M AU - John,RM AU - Fisher,AG DO - 10.1016/j.celrep.2017.01.010 EP - 1099 PY - 2017/// SN - 2211-1247 SP - 1090 TI - Visualizing changes in Cdkn1c expression links early life adversity to imprint mis-regulation in adults T2 - Cell Reports UR - http://dx.doi.org/10.1016/j.celrep.2017.01.010 UR - https://www.sciencedirect.com/science/article/pii/S2211124717300281?via%3Dihub UR - http://hdl.handle.net/10044/1/43828 VL - 31 ER -