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• Journal article
  Rowlands CJ, Park D, Bruns OT, Piatkevich KD, Fukumura D, Jain RK, Bawendi MG, Boyden ES, So PTCet al., 2017,

  Wide-field three-photon excitation in biological samples

  , Light: Science and Applications, Vol: 6, Pages: 1-9, ISSN: 2047-7538

  Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.

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• Journal article
  Bruns OT, Bischof TS, Harris DK, Franke D, Shi Y, Riedemann L, Bartelt A, Jaworski FB, Carr JA, Rowlands CJ, Wilson MWB, Chen O, Wei H, Hwang GW, Montana DM, Coropceanu I, Achorn OB, Kloepper J, Heeren J, So PTC, Fukumura D, Jensen KF, Jain RK, Bawendi MGet al., 2017,

  Next-generation in vivo optical imaging with short-wave infrared quantum dots

  , NATURE BIOMEDICAL ENGINEERING, Vol: 1, ISSN: 2157-846X
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• Journal article
  Abshire JR, Rowlands CJ, Ganesan SM, So PTC, Niles JCet al., 2017,

  Quantification of labile heme in live malaria parasites using a genetically encoded biosensor

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 114, Pages: E2068-E2076, ISSN: 0027-8424
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• Journal article
  Park JK, Rowlands CJ, So PTC, 2017,

  Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device

  , MICROMACHINES, Vol: 8, ISSN: 2072-666X
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• Book chapter
  So PTC, Yew E, Rowlands C, 2016,

  Applications of Multiphoton Microscopy in Dermatology

  , Imaging in Dermatology, Pages: 241-268, ISBN: 9780128028384

  Multiphoton microscopy is an important imaging method for noninvasive visualization of dermal physiology and pathology. Multiphoton technology has certain advantages over other visualization techniques. Compared with ultrasound and optical coherence tomography, multiphoton microscopy offers submicron-level spatial resolution. Compared with optical wide-field imaging, multiphoton microscopy offers inherent three-dimensional resolved optical sectioning. Compared with confocal microscopy, multiphoton microscopy offers improved penetration depths in skin imaging of tissue endogenous and exogenous fluorophores. Advances in developing multiphoton microscopes with novel contrast mechanisms, such as second harmonic generation and stimulated Raman scattering, further allow visualization of skin morphology and function based on molecular-level signatures of biological molecules. Therefore multiphoton microscopy has found increasing usage in dermatology that will be reviewed in this chapter. This review covers the basic spectroscopic and imaging principles underlying multiphoton microscopy. Skin photophysics and photochemistry important for multiphoton imaging will be examined. We will review several classes of multiphoton microscope designs that are useful for dermal imaging. Finally, the application of multiphoton microscopy in imaging skin cancers and various other diseases will be covered. The uses of multiphoton imaging to study skin immuno-response, aging, and regeneration phenomena will be presented. Finally, the uses of multiphoton microscopy for analyzing the transdermal transport of drugs, cosmetics and other agents are summarized.

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  • Citations: 1
• Journal article
  Uzel SGM, Platt RJ, Subramanian V, Pearl TM, Rowlands CJ, Chan V, Boyer LA, So PTC, Kamm RDet al., 2016,

  Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units

  , SCIENCE ADVANCES, Vol: 2, ISSN: 2375-2548
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  • Citations: 92
• Journal article
  Rowlands CJ, Bruns OT, Bawendi MG, So PTCet al., 2015,

  Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs

  , JOURNAL OF BIOMEDICAL OPTICS, Vol: 20, ISSN: 1083-3668
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  • Citations: 5
• Conference paper
  Xue Y, Rowlands CJ, So PTC, 2015,

  Parallel and Flexible Imaging using Two-photon RESOLFT Microscopy with Spatial Light Modulator Control

  , Conference on Multiphoton Microscopy in the Biomedical Sciences XV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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  • Citations: 1
• Journal article
  Rowlands CJ, Wu J, Uzel SGM, Klein O, Evans CL, So PTCet al., 2014,

  3D-resolved targeting of photodynamic therapy using temporal focusing

  , Laser Physics Letters, Vol: 11, ISSN: 1612-2011

  A method for selectively inducing apoptosis in tumor nodules is presented, with close-to-cellular level resolution, using 3D-resolved widefield temporal focusing illumination. Treatment times on the order of seconds were achieved using Verteporfin as the photosensitizer, with doses of 30 μg ml−1 and below. Results were achieved on both 2D and 3D cell cultures, demonstrating that treatment was possible through approximately one hundred microns of dense tumor nodules.

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• Journal article
  Kong K, Rowlands CJ, Varma S, Perkins W, Leach IH, Koloydenko AA, Pitiot A, Williams HC, Notingher Iet al., 2014,

  Increasing the speed of tumour diagnosis during surgery with selective scanning Raman microscopy

  , JOURNAL OF MOLECULAR STRUCTURE, Vol: 1073, Pages: 58-65, ISSN: 0022-2860
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  • Citations: 9
• Journal article
  Yew E, Rowlands C, So PTC, 2014,

  Application of multiphoton microscopy in dermatological studies: A mini-review

  , JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES, Vol: 7, ISSN: 1793-5458
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  • Citations: 37
• Conference paper
  Rowlands CJ, Bruns OT, Franke D, Bawendi MG, So PTCet al., 2014,

  Near-infrared temporal focusing microscopy with quantum dot fluorophores

  The tissue penetration depth of temporal focusing is increased by utilizing longer excitation wavelengths combined with quantum dots possessing extremely high two-photon crosssections. Cerebral vasculature is imaged in a mouse brain in vivo.

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• Journal article
  So PTC, Yew EYS, Rowlands C, 2013,

  High-Throughput Nonlinear Optical Microscopy

  , BIOPHYSICAL JOURNAL, Vol: 105, Pages: 2641-2654, ISSN: 0006-3495
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  • Citations: 23
• Journal article
  Kong K, Rowlands CJ, Varma S, Perkins W, Leach IH, Koloydenko AA, Williams HC, Notingher Iet al., 2013,

  Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 15189-15194, ISSN: 0027-8424
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  • Citations: 129
• Journal article
  Rowlands CJ, Yew EYS, So PTC, 2013,

  Parallel super-resolution imaging

  , NATURE METHODS, Vol: 10, Pages: 709-710, ISSN: 1548-7091
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  • Citations: 1
• Journal article
  Rowlands CJ, So PTC, 2013,

  On the correction of errors in some multiple particle tracking experiments

  , APPLIED PHYSICS LETTERS, Vol: 102, ISSN: 0003-6951
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  • Citations: 6
• Journal article
  Rowlands CJ, Varma S, Perkins W, Leach I, Williams H, Notingher Iet al., 2012,

  Rapid acquisition of Raman spectral maps through minimal sampling: applications in tissue imaging

  , JOURNAL OF BIOPHOTONICS, Vol: 5, Pages: 220-229, ISSN: 1864-063X
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  • Citations: 27
• Conference paper
  Rowlands CJ, Notingher I, 2012,

  Selective sampling in hyperspectral raman micro-spectroscopy: Obtaining maximum data with minimal sampling time

  We present a new method for selectively sampling hyperspectral Raman maps in order to maximize the information that can be obtained for a given sample time. Example maps and comparisons with alternative methods are provided. © OSA 2012.

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• Journal article
  Kong K, Rowlands CJ, Elsheikha H, Notingher Iet al., 2012,

  Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

  , ANALYST, Vol: 137, Pages: 4119-4122, ISSN: 0003-2654
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  • Citations: 15
• Journal article
  Rowlands CJ, Elliott SR, 2011,

  Improved blind-source separation for spectra

  , JOURNAL OF RAMAN SPECTROSCOPY, Vol: 42, Pages: 1761-1768, ISSN: 0377-0486
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  • Citations: 7

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