spel

Interaction and Characterisation of Biologics (ICB)

ICB is a biologics subgroup of SPEL established in 2018 due to a growing number of researchers from biological backgrounds. Our research interests include using physicochemical approaches to advance medicine and bioprocess engineering.

The primary focus of our research is developing an improved fundamental understanding of protein phase behaviour and stability that is crucial for many areas within the biopharmaceutical industry. Our research has been driven by industrial interests within biologics discovery, downstream processing and bioprocess formulation. We work with anything from monoclonal antibodies (immunotherapies that can be used for cancer and autoimmune diseases) to proteins associated with neurodegenerative diseases such as Alzheimer’s and Parkinson’s.

Research activities in our group include:
a) Interaction chromatography – Understanding how proteins interact with each other and with other proteins/ species to predict aggregation, solubility and crystallisation.

b) Inverse liquid protein chromatography – A novel way of characterising proteins and their surface hydrophobicity.

c) Freeze-drying of biologics – Studying the impact of moisture content and storage conditions on long term stability, protein activity and mechanical properties.

For more information about what we do or if you’re interested in working with us please contact Dr Sarah Hedberg.

Selected publications:

Hedberg, S. H. M., Lee, D., Mishra, Y., Haigh, J. M. and Williams, D. R. (2018). Mapping the mAb Aggregation Propensity Using Self-Interaction Chromatography as a Screening Tool. Analytical Chemistry, 90, 3878-3885. http://doi.org/10.1021/acs.analchem.7b04605

Duralliu, A., Matejtschuk, P. and Williams, D. R.  (2018). Humidity induced collapse in freeze dried cakes: A direct visualization study using DVS. European Journal of Pharmaceutics and Biopharmaceutics, 127, 29-36. https://doi.org/10.1016/j.ejpb.2018.02.003

Hedberg, S. H. M., Heng, J. Y. Y., Williams, D. R. and Liddell, J. M. (2016). Micro scale self-interaction chromatography of proteins: A mAb case-study. Journal of Chromatography A, 1434, 57-63. https://doi.org/10.1016/j.chroma.2015.12.034