Citation

BibTex format

@article{Vilar:2020:10.1038/s41598-020-75484-w,
author = {Vilar, Compte R and Reeh, K and Summers, P and Gould, I and Woscholski, R},
doi = {10.1038/s41598-020-75484-w},
journal = {Scientific Reports},
title = {Design, synthesis and evaluation of a tripodal receptor for phosphatidylinositol phosphates},
url = {http://dx.doi.org/10.1038/s41598-020-75484-w},
volume = {10},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Phosphatidylinositol phosphates (PIPs) are membrane phospholipids that play crucial roles in a wide range of cellular processes. Their function is dictated by the number and positions of the phosphate groups in the inositol ring (with seven different PIPs being active in the cell). Therefore, there is significant interest in developing small-molecule receptors that can bind selectively to these species and in doing so affect their cellular function or be the basis for molecular probes. However, to date there are very few examples of such molecular receptors. Towards this aim, herein we report a novel tripodal molecule that acts as receptor for mono- and bis-phosphorylated PIPs in a cell free environment. To assess their affinity to PIPs we have developed a new cell free assay based on the ability of the receptor to prevent alkaline phosphatase from hydrolysing these substrates. The new receptor displays selectivity towards two out of the seven PIPs, namely PI(3)P and PI(3,4)P2. To rationalise these results, a DFT computational study was performed which corroborated the experimental results and provided insight into the host–guest binding mode.
AU - Vilar,Compte R
AU - Reeh,K
AU - Summers,P
AU - Gould,I
AU - Woscholski,R
DO - 10.1038/s41598-020-75484-w
PY - 2020///
SN - 2045-2322
TI - Design, synthesis and evaluation of a tripodal receptor for phosphatidylinositol phosphates
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/s41598-020-75484-w
UR - http://hdl.handle.net/10044/1/84828
VL - 10
ER -