@article{Vilar:2021:10.1002/anie.202109349,
author = {Vilar, R and Priessner, M and Summers, PA and Lewis, B and Sastre, M and Ying, L and Kuimova, MK},
doi = {10.1002/anie.202109349},
journal = {Angewandte Chemie International Edition},
pages = {23148--23153},
title = {Selective detection of Cu+ ions in live cells via fluorescence lifetime imaging microscopy.},
url = {http://dx.doi.org/10.1002/anie.202109349},
volume = {60},
year = {2021}
}

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TY  - JOUR
AB  - Copper is an essential trace element in living organisms with its levels and localisation being carefully managed by the cellular machinery. However, if misregulated, deficiency or excess of copper ions can lead to several diseases. Therefore, it is important to have reliable methods to detect, monitor and visualise this metal in cells. Herein we report a new optical probe based on BODIPY, which shows a switch-on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions. More interestingly, binding to copper(I) leads to significant changes in the fluorescence lifetime of the new probe, which can be used to visualize copper(I) pools in lysosomes of live cells via fluorescence lifetime imaging microscopy (FLIM).
AU  - Vilar,R
AU  - Priessner,M
AU  - Summers,PA
AU  - Lewis,B
AU  - Sastre,M
AU  - Ying,L
AU  - Kuimova,MK
DO  - 10.1002/anie.202109349
EP  - 23153
PY  - 2021///
SN  - 1433-7851
SP  - 23148
TI  - Selective detection of Cu+ ions in live cells via fluorescence lifetime imaging microscopy.
T2  - Angewandte Chemie International Edition
UR  - http://dx.doi.org/10.1002/anie.202109349
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34379368
UR  - https://onlinelibrary.wiley.com/doi/10.1002/anie.202109349
UR  - http://hdl.handle.net/10044/1/91266
VL  - 60
ER  - 

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