FacilityUpdate

Our state-of-the-art multidisciplinary facilities help provide researchers and students the best collaborative and supported environment in which to learn and conduct novel and world leading research

All space within the department is shared, open and collaborative. It can fall into a number of subcategories including office and laboratory types; research specific space, core facility space and supportive space (PPE, Utility etc.)

All of our research facilities and teaching spaces are staffed and supported by a dedicated and highly skilled team of technical staff, providing an efficient and effective means of both delivering key technologies and ensuring the maintenance of a skills repository, which is essential in delivering our international leadingactivities.

Facility charges and costing can be accessed here - Queries to the relevant facility technical staff member or the Technical Operations Manager

Open to all departmental users are: our core Utilities spaces, (note: autoclave use requires training and induction)

and the various central PPE stores

SP8 - South Ken

See the source image

- DMi8CEL Inverted Motorised Research Microscope
- Optical Outfifit + Motorised & Coded DIC
- LED Epi Fluorescence Sola-SMII 365nm
- Transmitted Light Brightfifield Detector
- Scanning Stage - Including LASX Navigator Software
- Incubation Chamber - Whole Microscope Type
- Scan optics module HIVIS with Optical Field Rotation
- Tandem Scanner - FOV + 8KHz Resonant Scanner
- Three Channel Spectral Detection System (1 PMT + 2 Hybrid Detectors)
- Internal Detector Channel PMT 3
- 405 nm CW laser + Super Continuum White Light Laser (470-670nm)
- Confocal Super-Resolution LAS X LIGHTNING - Included in Core LASX Software

SP5 - South Ken

SP5

Leica DMI 6000CS inverted microscope, motorized XY-stage and Z-drive motor focus (100 nm step size). A SuperZ Galvo stage (1500 µm travel range). A temperature and Co2 incubator for live cell imaging.

Objectives: 10x (dry), 20x (immersion), 40x (oil), 63x (glycerol)

Lasers:

- 100 mW Argon laser 458, 476, 488, 496 and 514 nm

- 2 mW HeNe 594 nm

- 10 mW HeNe 633 nm

Stellaris

Confocal Microscope Platform STELLARIS

To publish cutting-edge research, you need to be able to see more, to try novel applications and to be able to collect reliable data. Our mission is to be your partner in microscopy and to empower you to drive progress in science. With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

SP8 - White City

SPECIFICATIONS MICROSCOPES Microscope stand Options Upright Leica DM6 CS Leica DM6 CFS Leica DM6 CFS w/o TL axis Inverted Leica DMi8 CS Leica DMi8 CEL Compact Leica DMi8 CEL Advanced VIBRATION ISOLATION Isolation options Specifications Anti-vibration table Yes (active/passive) MICROSCOPE STAGE Stage options Specifications Autofocus Optional reflection-based Adaptive Focus Control (AFC) for Leica DMi8 CS with 15 Hz sampling rate Image Based Autofocus for transmission light and fluorescence images. Can be combined with AFC Motorfocus Travel range depending on mechanics of microscope/ minimum step size 50 nm, adjustable in increments of < 4 nm Z galvanometer Selectable z modes (Galvo Flow/discrete steps) available, 1500 μm range/minimum step size 20 nm, adjustable in increments of < 1.5 nm Unique xzy scan mode for real-time xz slices, for setup of correction collar and for quick assessment of axial resolution CONTINUOUS WAVE LASERS Laser type Specifications VIS Solid state laser 40mW: 448 nm Solid state laser 20mW: 488 nm Solid state laser 20mW: 514 nm Solid state laser 20mW: 552 nm Solid state laser 30mW: 638 nm Diode 40mW: 442 nm Ar 65mW: 458, 476, 488, 496, 514 nm HeNe, 2mW: 594 nm HeNe, 10mW: 633 nm DPSS, 20mW

Thunder

Leica SP8 Specifications

ISU Leica SP8 Confocal System/White Light Laser/Falcon

Microscope:
-DMi8 CS inverted microscope; DIC
-Air Table / Compressor
-Super Z Galvo Stage; optional Universal or Multiwell plate inserts
-LMT200-T high precision scanning stage
-Adaptive Focus Control
-Turret Cooling
-LED brightfield light source
-Lumencor SOLA SE II white LED light source for wide-field fluorescence
-Leica DFC7000 T cooled color camera for non-confocal light and fluorescence imaging
-Transmitted light PMT
-Blue/green/red/far red filter cubes for wide-field fluorescence:
–DAPI bandpass filter cube. Excitation: BP 350/50, Emission: BP 460/50
–LED 470 filter cube. Excitation: 470/40, Dichroic: 495, Emission: 525/50
–RHOD bandpass filter cube. Excitation: BP 546/10, Emission: BP 585/40
–Y5 bandpass filter cube. Excitation: BP 620/60, Emission: BP 700/75

OBJECTIVES:
Summary: 5x; 10x; 20x-imm; 40x-oil; 40x-water; 63x-oil; 63x-glyc; 100x-oil.
1) HC PL FLUOTAR 5x/0.15. For use with and without coverglass, no immersion. Free working distance: 12.0 mm.
2) HC PL APO 10x/0.40 CS2. For use with 0.17 mm coverglass. Air immersion. Free working distance: 2.2 mm.
3) HC PL APO 20x/0.75 IMM CORR CS2. Correction collar for use with and without coverglass and use with water, glycerine and oil immersion. Free working distance: 0.67 mm (water immersion and 0.17 mm coverglass).
4) HC PL APO 40x/1.30 Oil CS2. Oil immersion. Free working distance: 0.24 mm.
5) HC PL APO 40x/1.10 W motCORR CS2. Motorized correction collar for adjustment of coverglass thickness from 0.14-0.18 mm, water and saltwater immersion and within the temperature range 20-40°C. Free working distance: 0.65 mm.
6) HC PL APO 63x/1.40 OIL CS2. Oil immersion. Free working distance: 0.14 mm.
7) HC PL APO 63x/1.30 GLYC CORR CS2. Glycerol immersion. Correction collar for use with coverglass (0.14-0.19 mm), glycerol immersion (80% glycerol / 20% water) and within the temperature range 20-40°C. Free working distance: 0.30 mm.
8) HC PL APO 100x/1.40 OIL CS2. Oil immersion. Free working distance: 0.13 mm.

TCS SP8 Confocal Scan Head
-Tandem Scanner
–Non-resonant (conventional/variable) Scanner: 1 – 1800 Hz (7 fps @ 512 x 512 px, 84 fps @ 512 x 16 px), line frequency up to 3600 lines/second (bidirectional), max. scan format 8192 x 8192 px, scan field 22 mm diag.
–Resonant Scanner (ultra-fast, reduced photodamage): 8000 Hz (28 fps @ 512 x 512 px, 290 fps @ 512 x 16 px), line frequency up to 16,000 lines/second (bidirectional), max. scan format 2496 x 2496 px, scan field 13 mm diag. Optical field rotation 200°.

-Notch filter set (provides additional suppression of excitation light) – NF 445; NF 488; NF 514; NF 458/514; NF 488/561/633; NF 594; NF 445/594

-5-channel filter?free prism detector system. Emission bands freely tunable with edge-positioning precision of 1nm. Lambda-Scan mode permits recording of spectral image series.
–Two PMTs (Hamamatsu R 9624 photomultiplier/ 40 MHz sampling/ detection range 400 – 800 nm)
–Three HyD hybrid Spectral detectors. Very low dark noise; Photon counting capability; Enables time gated detection.
-Programmable control panel

Excitation
-Continuous wave lasers
–405 nm Diode Laser (50 mW), DMOD
–Argon Laser (65 mW): 458 nm, 476 nm, 488 nm, 496 nm, 514 nm

-Pulsed lasers
–White light laser WLL2. Select up to 8 lines, individually tunable in steps of 1 nm from 470 to 670 nm, ~1.5 mW power per line. Pulsed, 80 MHz. 8 channel AOTF (Acousto-Optical-Tunable-Filter) for rapid modulation of laser intensity.
–Recording of two dimensional excitation-emission spectra supported by software for setup and display of 2D spectra.
–Lightgate function for avoidance of reflected laser light
–Pulsed 440 nm laser, LDH-P-C-440B. Suited to lifetime based measurements including FLIM. Software controlled selection of pulse frequency (40, 20, 10, and 5 MHz).

-LAS X Lightning Expert software for adaptive deconvolution

-FALCON (FAst Lifetime CONtrast) real time FLIM detection; FLIM-FRET analyzer.

-HPZ840 Workstation with Windows 10 Professional (64 bit) operating system. 2x Intel 4-core Xeon E5-2637 V4 3.5 GHz. 128 GByte RAM. NVIDIA Quadro GP100 16GB Reference GPU (3584 cores).
-Leica LAS X system software; LAS X MicroLab
-Software modules: LAS X 2D Analysis/Multi Channel; LAS X 3D Visualization; LAS X 3D Analysis

Schematic diagram of confocal detection setup — SP8 detector array: Emission light from the excited sample (left) passes through a prism, so that user-specified wavelength ranges can be transmitted to any of five detectors.

uCT scanner

ZEISS Xradia Context microCT

Your X-ray System for Today with Assurance for Tomorrow

ZEISS Xradia Context is a large field-of-view, non-destructive 3D X-ray micro-computed tomography system. With a robust stage and flexible software-controlled source/detector positioning, you can image large, heavy (25 kg), and tall samples in their full 3D context, as well as small samples with high resolution and detail.

Obtain 3D data on entire intact electronic components, large raw materials samples, or biological specimens.
Perform non-destructive failure analysis to identify internal defects without cutting your sample or workpiece.
Characterize and quantify performance-defining heterogeneities in your materials, like porosity, cracks, inclusions, defects, or multiple phases.
Perform 4D evolutionary studies, through ex situ treatment or in situ sample manipulation.
Connect to the ZEISS correlative microscopy environment and perform nondestructive 3D imaging to identify regions of interest for subsequent high resolution 2D or 3D electron microscopy imaging.

Genomics

We provide access to state-of-the-art DNA, RNA and Protein analysis instruments, methods and applications

Microbiology

bacterial stuff

PALM laser microdissection

Laser Microdissection with ZEISS PALM MicroBeam

The Patented Laser Technology for Contact-Free and Uncontaminated Specimen Collection

PALM MicroBeam uses a patented process to capture even the most demanding specimens - from cryosections, FFPE materials, native tissue like fresh plants, live cells and chromosomes - without contamination.

The Complete Workflow for Your Laser Microdissection Research

Laser Microdissection for Isolating High-Purity Tissue

PALM MicroBeam makes isolating uncontaminated source material simple. Precise detection, laser microdissection and patented laser transport allow you to obtain the homogenous analysis material necessary for meaningful scientific results.

Because analyses of gene expression patterns rely on exactly-separated analytical material, unwanted cells may alter your results and conceal the signals of the relevant cells. PALM MicroBeam prevents this by allowing you to define cells and tissue regions precisely, ensuring your results are exact and reproducible.

Histology

Histology Core Facility

Providing both the equipment and technical support, producing high-quality tissue sections for microscopy or digital imaging.

Histology supports a wide range of research projects within the Faculty across most research groups.

Our state-of-the-art facility is housed within the A.V. Hill building, room G.013.

Handling of tissue prior to and during histological techniques is a key step in obtaining good images. Before you begin your project we encourage you to discuss your requirements with us. We provide advice and protocols on the best methods for necropsy, tissue preparation, preservation, processing and staining in order to achieve the best images from your research tissue samples.

We provide training and support for all histological procedures from tissue/cell acquisition, cryotomy techniques, fixation, processing, sectioning and staining, including standard and special histological, immunohistochemical (IHC) and molecular pathology techniques such as in situ hybridization (ISH) staining protocols.

The equipment is available for use by trained staff and students via an online booking system. You will be required to be registered on PPMS

Mol. Bioengineering teaching lab

RCS1

See the source image

TissueCyte

TissueCyte 1000

An automated system from TissueVision Inc. capable of two-photon, sub-micron resolution imaging of entire small organs, ex vivo. A mouse brain, or similarly sized organ, can serially imaged over several days to generate three-dimensional data that can be used to map structures of interest such as cells, plaques or white/grey matter morphology.

PI charge out facility. Charged per hour of usage. Contact for more information.

Access and training

you can get access by

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Contacts

Miguel

Marta

Sharad

Olivier

Nana

B220

Bessemer B200 space

Uren L6

combined Electronics and mechanics - laser cuttitng and 3D printing

External - Hackspace

Mechanical workshop

workshop

Location: B220, LG04 Lower Ground Floor Royal School of Mines

Contact: Ji Yoon, Mechanical workshop Technician

Russell Stracey, Workshop Supervisor

Shared with Earth Sciences and Materials departments the main function of the Royal School of Mines mechanical workshop is to provide mechanical engineering support for teaching and research. Working closely with research staff and students, technicians help to prepare samples and design, develop and manufacture prototype experimental equipment from a wide range of engineering materials for use in teaching and research. The activities performed are:

- CNC (Computer Numerical Control)

- machining Spark & Wire EDM (Electro Discharge Machining)

- Grinding of metals and ceramics

Bessemer B220 is a workshop which is primarily used for projects/timetabled work. The lab has been fitted out for tensil testing, 3D printing, laser cutting etc. but is also suited for small mechanical work (drilling, sawing, gluing etc). Students are able to reserve a bench for projects.

Link to workshop site for more information

Electrical

Location RSM 3.19, RSM 4.22/4.25 4th floor Royal School of Mines.

Contact: Paschal Egan, Tarik Malik

Niraj Kanabar (White City)

RSM 4.22 is an undergraduate electronic teaching laboratory which facilitates the practical aspects of modules as well as project work.

Research groups must utilise this dedicated electronics facility rather than solder in their own laboratory space (unless risk assessments and suitable fume extracts are purchased and appropriately maintained).

Dual Power Supplies

Reflow controllers

Reflow Station

Signal Generator

Soldering stations

Access

Access is granted via training and development

Contacts

Paschal

Niraj

Bionanofabrication suite

White City.......

SK prototyping core

Masks etc.

Imperial College London

Imperial Alert

Coronavirus (COVID-19) updates: Safety information for academic year 2021-22

Department of Bioengineering