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Journal articleSwitzer A, Burchell L, McQuail J, et al., 2022,
The Adaptive Response to Long-Term Nitrogen Starvation in <i>Escherichia</i> <i>coli</i> Requires the Breakdown of Allantoin (vol 202, e00172-20, 2020)
, JOURNAL OF BACTERIOLOGY, Vol: 204, ISSN: 0021-9193 -
Journal articleZhong Q, Chatterjee S, Choudhary JS, et al., 2022,
EPEC-induced activation of the Ca<SUP>2+</SUP> transporter TRPV2 leads to pyroptotic cell death
, MOLECULAR MICROBIOLOGY, Vol: 117, Pages: 480-492, ISSN: 0950-382X- Author Web Link
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- Citations: 2
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Journal articleAmin H, Ilangovan A, Costa TRD, 2022,
Publisher Correction: Architecture of the outer-membrane core complex from a conjugative type IV secretion system.
, Nature Communications, Vol: 13, Pages: 1-1, ISSN: 2041-1723 -
Journal articleJanssens J, Aibar S, Taskiran II, et al., 2022,
Decoding gene regulation in the fly brain
, NATURE, Vol: 601, Pages: 630-+, ISSN: 0028-0836- Author Web Link
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- Citations: 40
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Conference paperMartinez-Gili L, Gordon H, Blad W, et al., 2022,
Gut bacteria composition and familiality echo Inflammatory Bowel Disease type and pathological spectrum
, 17th Congress of ECCO, Publisher: Oxford University Press, Pages: I601-I602, ISSN: 1873-9946BackgroundInflammatory bowel disease (IBD) aetiology encompasses genetic and environmental factors. Twin studies provide valuable insights to the familial degree (shared genetics and environment) of observed phenotypes. We characterised the gut bacterial composition of twins with IBD to find taxa associated with disease and estimate their familiality.MethodsFaecal samples were collected from 88 monozygotic (MZ) and dizygotic (DZ) twin pairs concordant or discordant for Crohn’s disease (CD; 26 MZ; 19 DZ) or ulcerative colitis (UC; 16 MZ; 27 DZ). The 16S rRNA gene was sequenced and amplicon sequence variants (ASV) generated. ANCOM software was used to assess differences in IBD vs. non-IBD, stratifying by disease type (CD/UC) and adjusting for age, gender and smoking. Twin pair and zygosity were added as random effects to estimate familiality, defined as percentage of variation due to common environment and genetics. IBD-affected twins were used for differences in disease location or treatment. Participants reporting antibiotic/probiotic treatment within the last 3 months or with a stoma/pouch were not included. In UC, surgery-naive patients were compared to an excluded subset who underwent ileostomy or pouch surgery without any recent antibiotic courses.ResultsDisease concordance in MZ twins was higher in CD (54%) than UC (19%). Alpha diversity was lower in CD, but not UC, and in ileostomy and pouch vs. surgery-naive UC. Principal component analysis showed that CD-affected twins clustered apart from non-IBD ones (Figure 1A). Familiality was lower in CD, with 5% of ASVs having familiality > 50%, compared to 17% in UC (Figure 1B). Two Lachnospirales order ASVs were less abundant in UC, while 15 ASVs from Clostridia, Bacteroidia, Bacilli and Coriobacteriia classes differentiated CD from non-IBD. Firmicutes were higher in CD (β= 0.95; 95%CI [0.34,1.56]), while no phyla changed in UC. Veillonella, Barnesiella, Faecalimonas and Holdemania genera had opposite t
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Journal articleFurniss RCD, Kaderabkova N, Barker D, et al., 2022,
Breaking antimicrobial resistance by disrupting extracytoplasmic protein folding
, ELIFE, Vol: 11, ISSN: 2050-084X- Author Web Link
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- Citations: 8
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Journal articleNuh A, Ramadan N, Schelenz S, et al., 2022,
Comparative Evaluation of MIRONAUT-AM and CLSI broth microdilution method for antifungal susceptibility testing of <i>Aspergillus species</i> against four commonly used antifungals (vol 81, myaa020, 2018)
, MEDICAL MYCOLOGY, Vol: 60, ISSN: 1369-3786 -
Journal articleMcQuail J, Carpousis AJ, Wigneshweraraj S, 2022,
The association between Hfq and RNase E in long-term nitrogen-starved <i>Escherichia coli</i>
, MOLECULAR MICROBIOLOGY, Vol: 117, Pages: 54-66, ISSN: 0950-382X- Author Web Link
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- Citations: 9
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Journal articleHughes DA, Archangelidi O, Coates M, et al., 2022,
Clinical characteristics of Pseudomonas and Aspergillus co-infected cystic fibrosis patients: A UK registry study
, JOURNAL OF CYSTIC FIBROSIS, Vol: 21, Pages: 129-135, ISSN: 1569-1993- Author Web Link
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- Citations: 3
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Journal articleMcCarthy AJ, Taylor PW, 2022,
Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing.
, Methods Mol Biol, Vol: 2377, Pages: 199-213Transposon-directed insertion site sequencing (TraDIS) combines random transposon mutagenesis and massively parallel sequencing to shed light on bacterial gene function on a genome-wide scale and in a high-throughput manner. The technique has proven to be successful in the determination of the fitness contribution of every gene under specific conditions both in vitro and in vivo. In this contribution, we describe the procedure used for the identification of Escherichia coli K1 genes essential for in vitro growth, survival in pooled human serum and gastrointestinal colonisation in a rodent model of neonatal invasive infection. TraDIS has broad application for systems-level analysis of a wide range of pathogenic, commensular and saprophytic bacteria.
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