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• Journal article
  Bill R, Byrne B, 2013,

  Foreword for EDICT Special Edition, volume 2

  , MOLECULAR MEMBRANE BIOLOGY, Vol: 30, Pages: 113-113, ISSN: 0968-7688
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• Journal article
  Curry S, 2013,

  Open access: brave new world requires bravery

  , Insights, Vol: 26, Pages: 22-27, ISSN: 2048-7754
  • Open Access Link
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• Journal article
  Faunce TA, Lubitz W, Rutherford AWB, MacFarlane DR, Moore GF, Yang P, Nocera DG, Moore TA, Gregory DH, Fukuzumi S, Yoon KB, Armstrong FA, Wasielewski MR, Styring Set al., 2013,

  Energy and environment policy case for a global project on artificial photosynthesis

  , ENERGY & ENVIRONMENTAL SCIENCE, Vol: 6, Pages: 695-698, ISSN: 1754-5692
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  • Citations: 228
• Journal article
  Chappell J, Jensen K, Freemont PS, 2013,

  Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

  , Nucleic Acids Research, Vol: 41, Pages: 3471-3481, ISSN: 0305-1048

  A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which are inherently time-consuming. To address this, we have validated a completely in vitro approach for the characterization of DNA regulatory elements using Escherichia coli extract cell-free systems. Importantly, we demonstrate that characterization in cell-free systems correlates and is reflective of performance in vivo for the most frequently used DNA regulatory elements. Moreover, we devise a rapid and completely in vitro method to generate DNA templates for cell-free systems, bypassing the need for DNA template generation and amplification from living cells. This in vitro approach is significantly quicker than current characterization methods and is amenable to high-throughput techniques, providing a valuable tool for rapidly prototyping libraries of DNA regulatory elements for synthetic biology.

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• Journal article
  Bill R, Byrne B, 2013,

  Introduction to EDICT volume 1

  , MOLECULAR MEMBRANE BIOLOGY, Vol: 30, Pages: 2-2, ISSN: 0968-7688
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• Journal article
  Porta C, Xu X, Loureiro S, Paramasivam S, Ren J, Al-Khalil T, Burman A, Jackson T, Belsham GJ, Curry S, Lomonossoff GP, Parida S, Paton D, Li Y, Wilsden G, Ferris N, Owens R, Kotecha A, Fry E, Stuart DI, Charleston B, Jones IMet al., 2013,

  Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity

  , JOURNAL OF VIROLOGICAL METHODS, Vol: 187, Pages: 406-412, ISSN: 0166-0934
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  • Citations: 41
• Journal article
  Ruggiero A, Marchant J, Squeglia F, Makarov V, De Simone A, Berisio Ret al., 2013,

  Molecular determinants of inactivation of the resuscitation promoting factor B from Mycobacterium tuberculosis

  , JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, Vol: 31, Pages: 195-205, ISSN: 0739-1102
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  • Citations: 26
• Journal article
  Xu Y, Matthews S, 2013,

  MAP-XSII: an improved program for the automatic assignment of methyl resonances in large proteins

  , JOURNAL OF BIOMOLECULAR NMR, Vol: 55, Pages: 179-187, ISSN: 0925-2738
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  • Citations: 30
• Journal article
  Solomon A, Smyth E, Mitha N, Pitchford S, Vydyanath A, Luther PK, Thorley AJ, Tetley TD, Emerson Met al., 2013,

  Induction of platelet aggregation after a direct physical interaction with diesel exhaust particles

  , JOURNAL OF THROMBOSIS AND HAEMOSTASIS, Vol: 11, Pages: 325-334, ISSN: 1538-7933
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  • Citations: 20
• Journal article
  Wang GD, Heng JYY, Williams DR, 2013,

  Dilatometry of powder compacts - Characterizing amorphous-crystalline transformations

  , POWDER TECHNOLOGY, Vol: 236, Pages: 12-16, ISSN: 0032-5910
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  • Citations: 4
• Journal article
  Khoo JY, Shah UV, Schaepertoens M, Williams DR, Heng JYYet al., 2013,

  Process-induced phase transformation of carbamazepine dihydrate to its polymorphic anhydrates

  , POWDER TECHNOLOGY, Vol: 236, Pages: 114-121, ISSN: 0032-5910
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  • Citations: 19
• Journal article
  Delmas T, Shah UV, Roberts MM, Williams DR, Heng JYYet al., 2013,

  Crystallisation of the orthorhombic form of acetaminophen: Combined effect of surface topography and chemistry

  , POWDER TECHNOLOGY, Vol: 236, Pages: 24-29, ISSN: 0032-5910
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  • Citations: 16
• Conference paper
  Zhao Z, Rutherford AW, Moser CC, Dutton PLet al., 2013,

  Photosynthetic Reaction Center Performance under Physiologically Relevant Energetic Changes

  , 57th Annual Meeting of the Biophysical-Society, Publisher: CELL PRESS, Pages: 489A-489A, ISSN: 0006-3495
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• Journal article
  Simon AC, Simpson PJ, Goldstone RM, Krysztofinska EM, Murray JW, High S, Isaacson RLet al., 2013,

  Structure of the Sgt2/Get5 complex provides insights into GET-mediated targeting of tail-anchored membrane proteins

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 1327-1332, ISSN: 0027-8424
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  • Citations: 25
• Journal article
  Saito K, Rutherford AW, Ishikita H, 2013,

  Mechanism of proton-coupled quinone reduction in Photosystem II

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 954-959, ISSN: 0027-8424
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  • Citations: 95
• Journal article
  Mesquita FS, Holden DW, Rolhion N, 2013,

  Lack of Effect of the Salmonella Deubiquitinase SseL on the NF-kappa B Pathway

  , PLOS ONE, Vol: 8, ISSN: 1932-6203
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  • Citations: 16
• Journal article
  Metifiot M, Maddali K, Johnson BC, Hare S, Smith SJ, Zhao XZ, Marchand C, Burke TR, Hughes SH, Cherepanov P, Pommier Yet al., 2013,

  Activities, Crystal Structures, and Molecular Dynamics of Dihydro-1H-isoindole Derivatives, Inhibitors of HIV-1 lntegrase

  , ACS CHEMICAL BIOLOGY, Vol: 8, Pages: 209-217, ISSN: 1554-8929
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  • Citations: 39
• Journal article
  Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SMet al., 2013,

  Architecture of the major component of the type III secretion system export apparatus

  , NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 20, Pages: 99-U126, ISSN: 1545-9993
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  • Citations: 160
• Journal article
  Bebeacua C, Lai L, Vegge CS, Brondsted L, van Heel M, Veesler D, Cambillau Cet al., 2013,

  Visualizing a Complete Siphoviridae Member by Single-Particle Electron Microscopy: the Structure of Lactococcal Phage TP901-1

  , JOURNAL OF VIROLOGY, Vol: 87, Pages: 1061-1068, ISSN: 0022-538X
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  • Citations: 47
• Journal article
  Hohenester E, Yurchenco PD, 2013,

  Laminins in basement membrane assembly

  , CELL ADHESION & MIGRATION, Vol: 7, Pages: 56-63
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  • Citations: 262
• Journal article
  Thapper A, Styring S, Saracco G, Rutherford AW, Robert B, Magnuson A, Lubitz W, Llobet A, Kurz P, Holzwarth A, Fiechter S, De Groot H, Campagna S, Braun A, Bercegol H, Artero Vet al., 2013,

  Artificial photosynthesis for solar fuels - An evolving research field within AMPEA, a joint programme of the European energy research alliance

  , Green, Vol: 3, Pages: 43-57, ISSN: 1869-876X

  On the path to an energy transition away from fossil fuels to sustainable sources, the European Union is for the moment keeping pace with the objectives of the Strategic Energy Technology-Plan. For this trend to continue after 2020, scientific breakthroughs must be achieved. One main objective is to produce solar fuels from solar energy and water in direct processes to accomplish the efficient storage of solar energy in a chemical form. This is a grand scientific challenge. One important approach to achieve this goal is Artificial Photosynthesis. The European Energy Research Alliance has launched the Joint Programme "Advanced Materials & Processes for Energy Applications" (AMPEA) to foster the role of basic science in Future Emerging Technologies. European researchers in artificial photosynthesis recently met at an AMPEA organized workshop to define common research strategies and milestones for the future. Through this work artificial photosynthesis became the first energy research sub-field to be organised into what is designated "an Application" within AMPEA. The ambition is to drive and accelerate solar fuels research into a powerful European field - in a shorter time and with a broader scope than possible for individual or national initiatives. Within AMPEA the Application Artificial Photosynthesis is inclusive and intended to bring together all European scientists in relevant fields. The goal is to set up a thorough and systematic programme of directed research, which by 2020 will have advanced to a point where commercially viable artificial photosynthetic devices will be under development in partnership with industry.

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  • Citations: 66
• Book chapter
  Goers L, Kylilis N, Tomazou M, Wen KY, Freemont P, Polizzi Ket al., 2013,

  Engineering Microbial Biosensors

  , MICROBIAL SYNTHETIC BIOLOGY, Editors: Harwood, Wipat, Publisher: ELSEVIER ACADEMIC PRESS INC, Pages: 119-156, ISBN: 978-0-12-417029-2
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  • Citations: 9
• Journal article
  Chappell J, Freemont P, 2013,

  In Vivo and In Vitro Characterization of sigma(70) Constitutive Promoters by Real-Time PCR and Fluorescent Measurements

  , SYNTHETI C BIOLOGY, Vol: 1073, Pages: 61-74, ISSN: 1064-3745
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  • Citations: 3
• Book
  Matthews S, Heise H, 2013,

  Modern NMR Methodology Preface

  , Publisher: SPRINGER-VERLAG BERLIN
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• Journal article
  Boehm CR, Freemont PS, Ces O, 2013,

  Design of a prototype flow microreactor for synthetic biology in vitro

  , LAB ON A CHIP, Vol: 13, Pages: 3426-3432, ISSN: 1473-0197
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  • Citations: 23
• Journal article
  Kaucikas M, Warren M, Michailovas A, Antanavicius R, van Thor JJet al., 2013,

  Beam patterns in OPO employing walk-off compensating BBO crystals

  , Laser Physics, Pages: 1-6
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• Book chapter
  Choudhury HG, Beis K, 2013,

  Tellurite-Resistance Protein TehA from Escherichia coli

  , Encyclopedia of Metalloproteins, Editors: Kretsinger, Uversky, Permyakov, Publisher: Springer, ISBN: 978-1-4614-1532-9
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• Book chapter
  Choudhury HG, Beis K, 2013,

  Tellurite-Detoxifying Protein TehB from Escherichia coli

  , Encyclopedia of Metalloproteins, Editors: Kretsinger, Uversky, Permyakov, Publisher: Springer, ISBN: 978-1-4614-1532-9
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• Journal article
  Juozapavicius M, Kaucikas M, Dimitrov SD, Barnes PRF, van Thor JJ, O'Regan BCet al., 2013,

  Evidence for “Slow” Electron Injection in Commercially Relevant Dye-2 Sensitized Solar Cells by Vis−NIR and IR Pump−Probe Spectroscopy

  , J Phys Chem C

  We present femtosecond to nanosecond transient absorption (TA)data on electron injection in dye-sensitized solar cells (DSSCs) fabricated with lowvolatility, commercially relevant electrolytes, with and without added lithium.Results are shown over an extended time range (300 fs−6.3 ns) and extendedwavelength range (800−1400 nm) for both N719 and C106 dyes. Kinetics weremeasured on both TiO2 and noninjecting ZrO2. Using the latter, we havedetermined the spectra and absorption coefficient of N719* across the wavelengthrange. We find an isosbestic point in the TA spectra on TiO2 near 900 nm for allcells, existing from <1 ps to >1 ns. We show how measurements near this isosbesticpoint can give a false impression of uniformly femtosecond injection dynamics inDSSCs. Comparison of dynamics measured at 1200 nm with mid-IR transient absorption measured at 5100 nm confirms amajority proportion of slow (>10 ps) electron injection in these commercially relevant cells. We also comment on a recentpublication which appears to directly contradict the results we present.

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• Journal article
  Warren M, Kaucikas M, Fitzpatrick A, Champion P, Sage JT, van Thor JJet al., 2013,

  Ground state proton transfer in the photoswitching reactions of the fluorescent protein Dronpa

  , Nature Communications
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