@article{Kim:2012,
author = {Kim, TW and Lee, JH and Choi, J and Kim, KH and van, Wilderen LJ and Guerin, L and Kim, Y and Jung, YO and Yang, C and Kim, J and Wulff, M and van, Thor JJ and Ihee, H},
journal = {J. Am. Chem. Soc.},
title = {Protein Structural Dynamics of Photoactive Yellow Protein in Solution Revealed by Pump−Probe X-ray Solution Scattering.},
url = {http://dx.doi.org/10.1021/ja210435n},
year = {2012}
}

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TY  - JOUR
AB  - Photoreceptor proteins play crucial roles in receiving light stimuli that give rise to the responses required for biological function.   However, structural characterization of conformational transition of the   photoreceptors has been elusive in their native aqueous environment, even for   a prototype photoreceptor, photoactive yellow protein (PYP). We employ   pump−probe X-ray solution scattering to probe the structural changes that   occur during the photocycle of PYP in a wide time range from 3.16 μs to 300   ms. By the analysis of both kinetics and structures of the intermediates, the   structural progression of the protein in the solution phase is vividly visualized.   We identify four structurally distinct intermediates and their associated five time constants and reconstructed the molecular   shapes of the four intermediates from time-independent, species-associated difference scattering curves. The reconstructed   structures of the intermediates show the large conformational changes such as the protrusion of N-terminus, which is restricted in   the crystalline phase due to the crystal contact and thus could not be clearly observed by X-ray crystallography. The protrusion of   the N-terminus and the protein volume gradually increase with the progress of the photocycle and becomes maximal in the final   intermediate, which is proposed to be the signaling state. The data not only reveal that a common kinetic mechanism is   applicable to both the crystalline and the solution phases, but also provide direct evidence for how the sample environment   influences structural dynamics and the reaction rates of the PYP photocycle.
AU  - Kim,TW
AU  - Lee,JH
AU  - Choi,J
AU  - Kim,KH
AU  - van,Wilderen LJ
AU  - Guerin,L
AU  - Kim,Y
AU  - Jung,YO
AU  - Yang,C
AU  - Kim,J
AU  - Wulff,M
AU  - van,Thor JJ
AU  - Ihee,H
PY  - 2012///
TI  - Protein Structural Dynamics of Photoactive Yellow Protein in Solution Revealed by Pump−Probe X-ray Solution Scattering.
T2  - J. Am. Chem. Soc.
UR  - http://dx.doi.org/10.1021/ja210435n
ER  - 

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