@article{Chappell:2013:nar/gkt052,
author = {Chappell, J and Jensen, K and Freemont, PS},
doi = {nar/gkt052},
journal = {Nucleic Acids Research},
pages = {3471--3481},
title = {Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology},
url = {http://dx.doi.org/10.1093/nar/gkt052},
volume = {41},
year = {2013}
}

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TY  - JOUR
AB  - A bottleneck in our capacity to rationally and predictably engineer biological systems is the limited number of well-characterized genetic elements from which to build. Current characterization methods are tied to measurements in living systems, the transformation and culturing of which are inherently time-consuming. To address this, we have validated a completely in vitro approach for the characterization of DNA regulatory elements using Escherichia coli extract cell-free systems. Importantly, we demonstrate that characterization in cell-free systems correlates and is reflective of performance in vivo for the most frequently used DNA regulatory elements. Moreover, we devise a rapid and completely in vitro method to generate DNA templates for cell-free systems, bypassing the need for DNA template generation and amplification from living cells. This in vitro approach is significantly quicker than current characterization methods and is amenable to high-throughput techniques, providing a valuable tool for rapidly prototyping libraries of DNA regulatory elements for synthetic biology.
AU  - Chappell,J
AU  - Jensen,K
AU  - Freemont,PS
DO  - nar/gkt052
EP  - 3481
PY  - 2013///
SN  - 0305-1048
SP  - 3471
TI  - Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkt052
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000318062600061&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/69622
VL  - 41
ER  - 

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