Citation

BibTex format

@article{Wobbe:2013:nar/gkt313,
author = {Wobbe, L and Nixon, PJ},
doi = {nar/gkt313},
journal = {Nucleic Acids Research},
pages = {6553--6567},
title = {The mTERF protein MOC1 terminates mitochondrial DNA transcription in the unicellular green alga Chlamydomonas reinhardtii},
url = {http://dx.doi.org/10.1093/nar/gkt313},
volume = {41},
year = {2013}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The molecular function of mTERFs (mitochondrialtranscription termination factors) has so far onlybeen described for metazoan members of theprotein family and in animals they control mitochondrialreplication, transcription and translation. Cells ofphotosynthetic eukaryotes harbour chloroplasts andmitochondria, which are in an intense cross-talk thatis vital for photosynthesis. Chlamydomonasreinhardtii is a unicellular green alga widely used asa model organism for photosynthesis research andgreen biotechnology. Among the six nuclear C.reinhardtii mTERF genes is mTERF-like gene ofChlamydomonas (MOC1), whose inactivation altersmitorespiration and interestingly also light-acclimationprocesses in the chloroplast that favour theenhanced production of biohydrogen. We show herefrom in vitro studies that MOC1 binds specifically to asequence within the mitochondrial rRNA-codingmodule S3, and that a knockout of MOC1 in themutant stm6 increases read-through transcription atthis site, indicating that MOC1 acts as a transcriptionterminator in vivo. Whereas the level of certainantisense RNA species is higher in stm6, the amountof unprocessed mitochondrial sense transcripts isstrongly reduced, demonstrating that a loss of MOC1causes perturbed mitochondrial DNA (mtDNA) expression.Overall, we provide evidence for the existenceof mitochondrial antisense RNAs in C. reinhardtiiand show that mTERF-mediated transcription terminationis an evolutionary-conserved mechanismoccurring in phototrophic protists and metazoans.
AU - Wobbe,L
AU - Nixon,PJ
DO - nar/gkt313
EP - 6567
PY - 2013///
SN - 1362-4962
SP - 6553
TI - The mTERF protein MOC1 terminates mitochondrial DNA transcription in the unicellular green alga Chlamydomonas reinhardtii
T2 - Nucleic Acids Research
UR - http://dx.doi.org/10.1093/nar/gkt313
UR - http://hdl.handle.net/10044/1/28016
VL - 41
ER -