@article{Pinzan:2015:10.1371/journal.pone.0143087,
author = {Pinzan, CF and Sardinha-Silva, A and Almeida, F and Lai, L and Lopes, CD and Lourenco, EV and Panunto-Castelo, A and Matthews, S and Roque-Barreira, MC},
doi = {10.1371/journal.pone.0143087},
journal = {PLOS One},
title = {Vaccination with Recombinant Microneme Proteins Confers Protection against Experimental Toxoplasmosis in Mice},
url = {http://dx.doi.org/10.1371/journal.pone.0143087},
volume = {10},
year = {2015}
}

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TY  - JOUR
AB  - Toxoplasmosis, a zoonotic disease caused by Toxoplasma gondii, is an important publichealth problem and veterinary concern. Although there is no vaccine for human toxoplasmosis,many attempts have been made to develop one. Promising vaccine candidates utilizeproteins, or their genes, from microneme organelle of T. gondii that are involved in theinitial stages of host cell invasion by the parasite. In the present study, we used differentrecombinant microneme proteins (TgMIC1, TgMIC4, or TgMIC6) or combinations of theseproteins (TgMIC1-4 and TgMIC1-4-6) to evaluate the immune response and protectionagainst experimental toxoplasmosis in C57BL/6 mice. Vaccination with recombinantTgMIC1, TgMIC4, or TgMIC6 alone conferred partial protection, as demonstrated byreduced brain cyst burden and mortality rates after challenge. Immunization with TgMIC1-4or TgMIC1-4-6 vaccines provided the most effective protection, since 70% and 80% ofmice, respectively, survived to the acute phase of infection. In addition, these vaccinatedmice, in comparison to non-vaccinated ones, showed reduced parasite burden by 59% and68%, respectively. The protective effect was related to the cellular and humoral immuneresponses induced by vaccination and included the release of Th1 cytokines IFN-γ and IL-12, antigen-stimulated spleen cell proliferation, and production of antigen-specific serumantibodies. Our results demonstrate that microneme proteins are potential vaccines againstT. gondii, since their inoculation prevents or decreases the deleterious effects of theinfection.
AU  - Pinzan,CF
AU  - Sardinha-Silva,A
AU  - Almeida,F
AU  - Lai,L
AU  - Lopes,CD
AU  - Lourenco,EV
AU  - Panunto-Castelo,A
AU  - Matthews,S
AU  - Roque-Barreira,MC
DO  - 10.1371/journal.pone.0143087
PY  - 2015///
SN  - 1932-6203
TI  - Vaccination with Recombinant Microneme Proteins Confers Protection against Experimental Toxoplasmosis in Mice
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0143087
UR  - http://hdl.handle.net/10044/1/28930
VL  - 10
ER  - 

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