Leverhulme Centre for Cellular Bionics Studentships

The Centre for Cellular Bionics is training a new generation of PhD graduates in the emerging field of Cellular Bionics to explore the science of fusing the living and non-living, transforming bioengineering and design.

Leverhulme Cellular Bionics Studentships Starting 2019

Advancing bacterial 3D printing for the production of next-generation bio-materials

Funded by the Leverhulme Doctoral Scholarship Programme in Cellular Bionics – 3 year PhD studentships - APPLY HERE

Supervisors: Dr Guy-Bart StanDr John HeapDr Connor Myant

Natural and engineered bacteria possess extraordinary biosynthetic capabilities. These can serve almost any application imaginable: from functionalised bacterial cellulose patches for antimicrobial wound dressing to bacterial self-healing concrete or the use of bacteria to produce nacre-inspired composite materials. The ability to harness such great manufacturing potential into customised designs with defined three-dimensional shape and composition remains, however, largely elusive.

Current 3D bacterial printing approaches rely on the use of scaffolds or conventional layer-by-layer additive manufacturing strategies to shape their designs, often resulting in unsophisticated structures with restricted geometries and monotonous physico-chemical and mechanical properties. In contrast, one-body yet heterogeneous composite materials with seamless transitions between disparate properties (functionally graded composite materials) have long been a holy grail for designers and engineers.

This project will employ a new 3D printing method, developed by the project supervisors, that is merging the fields of synthetic biology and 3D Printing. 3D Printing has enabled the creation of complex geometric structures previously unavailable to design engineers and scientists. However, this vast new pallet of object geometries has been limited to non-living materials. The ability to create bespoke biological structures directly from a 3D printing process has so far eluded us. The focus of this project is to challenge this limitation and open a new era of 3D bio-printing.

More specifically, this project will develop a novel 3D printing process that employs nature as the 3D printer, at a cellular level; whilst utilising engineering’s ability for highly accurate spatial control. It will build on ongoing efforts to bridge the gap between synthetic biology and 3D printing technology and will focus on further developing the necessary biological tools for the effective manufacturing of bio-materials in 3D. In the initial phases of the project, less-sophisticated, simple proof-of-concept structures will be generated. This will help identify and delimit expected and also unexpected challenges for the production of more complex composite materials. Next steps will include, but not be limited to, optimisation of biosynthetic pathways in the working chassis; finding appropriate external 3D control strategies to improve printing efficiency and accuracy; incorporation of biomolecular feedback control into genetic designs; development of efficient secretion systems and alternative “secretion” strategies (e.g. enzyme display for extracellular biosynthesis of one or more constituents of the composite material); bio-material engineering (e.g. through incorporation of bacterial amyloids) and functionalisation (e.g. silver nanoparticles); development and integration of mathematically-modelled gene expression systems into 3D printing software for true computer-guided bio-fabrication; etc. Finally, resultant next-generation 3D bio-materials will be analysed for their physico-chemical and mechanical behaviour and compared with existing bio-materials.

The successful development of this bio-printing technology could have significant impact in the medical field, biotechnology, and pharmaceutical industries. From the development of high throughput pharmaceutical screening equipment to 3D microfluidics and novel biomaterials. In addition the 3D printing technique offers a new paradigm in 3D manufacturing applications were the printing process is not limited to 2D planar layer stacks but rather a true 3D object.

The ideal candidate will have a background in some of these areas: bacterial synthetic biology (e.g. E. coli), genetic engineering, optogenetics, metabolic engineering, biomolecular feedback design and implementation, modelling in biology, computational modelling

The project will bring together a truly multidisciplinary team of synthetic biologists, bioengineers, and design engineers. The work will be support by a parallel project run by the supervisors; Dr Guy Bart Stan from the Department of Bioengineering, Dr John Heap from the Department of Life Sciences, and Dr Connor Myant from the Dyson School of Design Engineering. If you have any questions regarding the application please email Dr Guy-Bart Stan (g.stan@imperial.ac.uk).

SolarBioChip: development of a solar bio-battery for printed bioelectronics

Funded by the Leverhulme Doctoral Scholarship Programme in Cellular Bionics – 3 year PhD studentships - APPLY HERE

Supervisors: Prof Milo ShafferProf Peter Nixon | Prof Klaus Hellgardt

Applications are invited for a fully-funded PhD studentship to work on development of a thin-film biophotovoltaic system based on the interfacing of carbon nanotubes conductor with electrogenic cyanobacteria for bioelectricity generation and storage in next generation printed electronics, more specifically in bioelectronics.

We have recently described the development of a printed cyanobacterial biobattery with potential application as a biodegradable power supply for low-power devices including biosensors and printed bioelectronics (Sawa M, Fantuzzi A, Bombelli P, Howe CJ, Hellgardt K & Nixon PJ (2017) Electricity generation from digitally printed cyanobacteria. Nature Commun. 8, 1327). The biobattery is conveniently fabricated by a scalable inkjet method to print both cyanobacterial cells and carbon nanotube electrode surfaces on which the cells grow as a biofilm. In the light the printed device acts as a biophotovoltaic cell producing electrical current from electrons released from the cell during photosynthetic electron transport. Importantly, the biobattery can also produce electricity in the dark from the breakdown of stored products of photosynthesis, such as carbohydrate. The aim of this project is to improve the power output of the first-generation solar biobattery so that it can meet the power requirement for use in ultra-low-power microprocessors, which will be undertaken in collaboration with Arm Ltd. Areas for investigation include the development of novel cyanobacterial strains (Nixon), the testing of novel electrode materials for improved conductance and biocompatibility (Shaffer), the development of robust printing techniques and composite design (Hellgardt) and the testing of printed biobattery on powering an ultra-low-power Arm processor in collaboration with Arm Ltd. Ultimately, we aim to fabricate a semi-living electronic device powered by sunlight using cyanobacterial cells – the ‘solarbiochip’.