Citation

BibTex format

@article{Kumar:2011:10.1002/cphc.201000874,
author = {Kumar, S and Alibhai, D and Margineanu, A and Laine, R and Kennedy, G and McGinty, J and Warren, S and Kelly, D and Alexandrov, Y and Munro, I and Talbot, C and Stuckey, DW and Kimberly, C and Viellerobe, B and Lacombe, F and Lam, EW-F and Taylor, H and Dallman, MJ and Stamp, G and Murray, EJ and Stuhmeier, F and Sardini, A and Katan, M and Elson, DS and Neil, MAA and Dunsby, C and French, PMW},
doi = {10.1002/cphc.201000874},
journal = {ChemPhysChem: a European journal of chemical physics and physical chemistry},
pages = {609--626},
title = {FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ},
url = {http://dx.doi.org/10.1002/cphc.201000874},
volume = {12},
year = {2011}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - A fluorescence lifetime imaging (FLIM) technology platform intendedto read out changes in Fçrster resonance energy transfer(FRET) efficiency is presented for the study of protein interactionsacross the drug-discovery pipeline. FLIM provides arobust, inherently ratiometric imaging modality for drug discoverythat could allow the same sensor constructs to betranslated from automated cell-based assays through smalltransparent organisms such as zebrafish to mammals. To thisend, an automated FLIM multiwell-plate reader is described forhigh content analysis of fixed and live cells, tomographic FLIMin zebrafish and FLIM FRET of live cells via confocal endomicroscopy.For cell-based assays, an exemplar application readingout protein aggregation using FLIM FRET is presented, andthe potential for multiple simultaneous FLIM (FRET) readoutsin microscopy is illustrated.
AU - Kumar,S
AU - Alibhai,D
AU - Margineanu,A
AU - Laine,R
AU - Kennedy,G
AU - McGinty,J
AU - Warren,S
AU - Kelly,D
AU - Alexandrov,Y
AU - Munro,I
AU - Talbot,C
AU - Stuckey,DW
AU - Kimberly,C
AU - Viellerobe,B
AU - Lacombe,F
AU - Lam,EW-F
AU - Taylor,H
AU - Dallman,MJ
AU - Stamp,G
AU - Murray,EJ
AU - Stuhmeier,F
AU - Sardini,A
AU - Katan,M
AU - Elson,DS
AU - Neil,MAA
AU - Dunsby,C
AU - French,PMW
DO - 10.1002/cphc.201000874
EP - 626
PY - 2011///
SN - 1439-4235
SP - 609
TI - FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ
T2 - ChemPhysChem: a European journal of chemical physics and physical chemistry
UR - http://dx.doi.org/10.1002/cphc.201000874
UR - https://onlinelibrary.wiley.com/doi/full/10.1002/cphc.201000874
UR - http://hdl.handle.net/10044/1/76638
VL - 12
ER -