Publications

Publications from our Researchers

Several of our current PhD candidates and fellow researchers at the Data Science Institute have published, or in the proccess of publishing, papers to present their research.  

Citation

BibTex format

@article{Takahashi:2018:10.1183/13993003.02173-2017,
author = {Takahashi, K and Pavlidis, S and Ng, Kee Kwong F and Hoda, U and Rossios, C and Sun, K and Loza, M and Baribaud, F and Chanez, P and Fowler, SJ and Horvath, I and Montuschi, P and Singer, F and Musial, J and Dahlen, B and Dahlen, SE and Krug, N and Sandstrom, T and Shaw, DE and Lutter, R and Bakke, P and Fleming, LJ and Howarth, PH and Caruso, M and Sousa, AR and Corfield, J and Auffray, C and De, Meulder B and Lefaudeux, D and Djukanovic, R and Sterk, PJ and Guo, Y and Adcock, I and Chung, KF},
doi = {10.1183/13993003.02173-2017},
journal = {European Respiratory Journal},
title = {Sputum proteomics and airway cell transcripts of current and ex-smokers with severe asthma in U-BIOPRED: an exploratory analysis},
url = {http://dx.doi.org/10.1183/13993003.02173-2017},
volume = {51},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: Severe  asthma  patients  with  a  significant  smoking  history  have  airflow  obstruction  with reported neutrophilia.  We hypothesise that multi1omic analysis will enable the definition of smoking and ex1smoking severe asthma molecular phenotypes.Methods The  U1BIOPRED  severe  asthma  patients  containing  current1smokers  (CSA),  ex1smokers (ESA), non1smokers (NSA) and healthy non1smokers (NH) was examined.  Blood and sputum cell counts, fractional exhaled nitric oxide and spirometry were obtained. Exploratory proteomic analysis of sputum supernatants and transcriptomic analysis of bronchial brushings, biopsies and sputum cells was performed.   Results Colony stimulating factor (CSF)2 protein levels were increased in CSA sputum supernatants with azurocidin 1, neutrophil elastase and CXCL8 upregulated in ESA.  Phagocytosis and innate immune  pathways  were  associated  with  neutrophilic  inflammation  in  ESA.  Gene  Set  Variation Analysis  of  bronchial  epithelial  cell  transcriptome  from  CSA  showed  enrichment  of  xenobiotic metabolism, oxidative stress and endoplasmic reticulum stress compared to other groups.  CXCL5 and matrix metallopeptidase 12 genes were upregulated in ESA and the epithelial protective genes, mucin 2 and cystatin SN, were downregulated.   Conclusion Despite  little  difference  in  clinical  characteristics,  CSA  were  distinguishable  from  ESA subjects  at  the  sputum  proteomic  level  with  CSA  having  increased  CSF2  expression  and  ESA patients showed sustained loss of epithelial barrier processes.
AU  - Takahashi,K
AU  - Pavlidis,S
AU  - Ng,Kee Kwong F
AU  - Hoda,U
AU  - Rossios,C
AU  - Sun,K
AU  - Loza,M
AU  - Baribaud,F
AU  - Chanez,P
AU  - Fowler,SJ
AU  - Horvath,I
AU  - Montuschi,P
AU  - Singer,F
AU  - Musial,J
AU  - Dahlen,B
AU  - Dahlen,SE
AU  - Krug,N
AU  - Sandstrom,T
AU  - Shaw,DE
AU  - Lutter,R
AU  - Bakke,P
AU  - Fleming,LJ
AU  - Howarth,PH
AU  - Caruso,M
AU  - Sousa,AR
AU  - Corfield,J
AU  - Auffray,C
AU  - De,Meulder B
AU  - Lefaudeux,D
AU  - Djukanovic,R
AU  - Sterk,PJ
AU  - Guo,Y
AU  - Adcock,I
AU  - Chung,KF
DO  - 10.1183/13993003.02173-2017
PY  - 2018///
SN  - 0903-1936
TI  - Sputum proteomics and airway cell transcripts of current and ex-smokers with severe asthma in U-BIOPRED: an exploratory analysis
T2  - European Respiratory Journal
UR  - http://dx.doi.org/10.1183/13993003.02173-2017
UR  - http://hdl.handle.net/10044/1/57478
VL  - 51
ER  -
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