@article{Meredith:2010:10.1002/rcm.4314,
author = {Meredith, W and Gomes, RL and Cooper, M and Snape, CE and Sephton, MA},
doi = {10.1002/rcm.4314},
journal = {RAPID COMMUN MASS SP},
pages = {501--505},
title = {Hydropyrolysis over a platinum catalyst as a preparative technique for the compound-specific carbon isotope ratio measurement of C27 steroids},
url = {http://dx.doi.org/10.1002/rcm.4314},
volume = {24},
year = {2010}
}

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TY  - JOUR
AB  - Compound-specific stable carbon isotope analysis by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is an important method for the determination of the 13C/12C ratios of biomolecules such as steroids, for a wide range of applications. However, steroids in their natural form exhibit poor chromatographic resolution, while derivatisation adds carbon thereby corrupting the stable isotopic composition. Hydropyrolysis with a sulphided molybdenum catalyst has been shown to defunctionalise the steroids, while leaving their carbon skeleton intact, allowing for the accurate measurement of carbon isotope ratios. The presence of double bonds in unsaturated steroids such as cholesterol resulted in significant rearrangement of the products, but replacing the original catalyst system with one of platinum results in higher conversions and far greater selectivity. The improved chromatographic performance of the products should allow GC/C/IRMS to be applied to more structurally complex steroid hormones and their metabolites.
AU  - Meredith,W
AU  - Gomes,RL
AU  - Cooper,M
AU  - Snape,CE
AU  - Sephton,MA
DO  - 10.1002/rcm.4314
EP  - 505
PY  - 2010///
SN  - 1097-0231
SP  - 501
TI  - Hydropyrolysis over a platinum catalyst as a preparative technique for the compound-specific carbon isotope ratio measurement of C27 steroids
T2  - RAPID COMMUN MASS SP
UR  - http://dx.doi.org/10.1002/rcm.4314
UR  - http://www3.interscience.wiley.com/cgi-bin/fulltext/123264239/PDFSTART
VL  - 24
ER  - 

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