Facility

The Electron Microscopy Centre is setup for single particle analysis (SPA) and cryo-electron tomography (cryoET) ad is sed prodomiatly for te screeing of complex membrane proteins and cell membrane tissues.

The facility is equipped with 4 tranmission electron microscopes:-

  • Tecnia 12spirit is used for low resolution negative stain imaging and sample optimisation and new user training.
  • Tecnia 12 Twin in the facility is used for cryoEM sample optimisation ad cryoEM training.
  • Tecani F20 SFEG is for high resolution screening and small dataset collection (side entry system).
  • Glacios XFEG is the facility flag ship and is for high resolution screening of autogrids and data collection ad can screen multiple grids atonomously (Autoloader system).

The facility has a sample preparation labs, equipped stain their samples or freeze their samples in vitrified ice using The facility also has Vitrobot for sample vitrification and several cryo transfer holders (e.g Gatan 626 and 914) with and without high tilt capabilities for tomography and single particle data collection, respectively.


Access

Please note the EM facility is setup for single particle analysis (SPA) and is equipped for the following applications; Negative stain, CryoEM and Tomography of single particles and cell membrane tissues.  Please contact the facility manager if you wish to perform other other application outside of this scope.

To use the facility for your research project?

  • Imperial College investigators/groups within the Department of Life Science, please register here on our PPMS booking system for access to the facility.
  • All o#ig# ther Imperial College or external interested parties, please contact the Facility Manager to discuss your project and the options available to you to access the facility.

Transmission Electron Microscopes in the Centre

TEM - Glacios (XFEG)

Microscope

200kV cryo-transmission electron microscope (TEM) use for high resolution structural analysis (single particle and tomography) of protein complexes and cell membrane specimens, using a variety of applications and acquisition software to generate the images and data collections.  This microscope also has 12 grid autoloading system an automated LN2 filling to accomadate autonmous data collections.

NEWS - recently been awarded funding for the new Selectris energy filter.

Camera

  • FALCON 4 - Direct Electron Detector
  • CETA D - CMOS camera, diffraction scintilator
Glacios

Applications

  • SPA (Single particle analyses) - EPU automated data collection
  • CryoET - Leginon automated data collection or Tomo 4
  • MicroED - microcrystal electron diffraction
  • Phase Plate

Software

  • EPU version 2.0
  • Leginon (cryoET)
  • Tomo5
  • Sherpa (AutoCTF)
  • Maps
  • Athena (live data validation)

Holders

Autoloader system holds 12 autogrids.

Tecnia F20

Microscope

200kV cryo-transmission electron microscope (TEM) use for high resolution structural analysis (single particle and tomography) of protein complexes and cell membrane specimens, using a variety of applications and acquisition software to generate the images and data collections.  This microscope also has an automated LN2 filling robot to allow over-night cryo-tomography data collections.gug

Camera

  • CMOS 16 megapixel 'Direct Electron Detector' (Falcon II)
  • CCD 2K camera (Gatan Orius SC200)

Applications

  • SPA (Single particle analyses) - EPU automated data collection
  • Cryo-tomography - Leginon automated data collection
  • Diffraction

Software

  • EPU
  • Leginon (cryoET)
  • Serial EM - Data acquisition software (to be installed)
  • Digital Micrograph - Orius camera software
  • TIA

Holders

  • Gatan 626 cryo-holder
  • Gatan 914 tomography holder

FEI Tecnai G2 Spirit TWIN

Microscope

This 120kV TEM is a popular microscope use prodominantly for negative stain screening and training.  It's also cryo-capable.spirit

Specification

  • FEI 2K eagle camera
  • LAB6 (standard 90 degrees)
  • TIA
  • Low Dose (CryoEM)

Tecnai T12 twin (LAB6)

Microscope

This 120kV twin lens microscope is used for cryo-TEM screening with 626 holder for checking vitreous ice thickness, contamination, particle concentration and conformation. t12

Specification

  • CCD camera (F216, TVIPS)
  • Leginon data collection.
  • EMMenu 4
  • LAB6 filament (60 degrees)
  • Low Dose option
  • Gatan 626 cryo-holder
  • Cryo-TEM screening