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• Journal article
  Ellis T, Evans DA, Martin CRH, Hartley JAet al., 2007,

  A 96-well DNase I footprinting screen for drug-DNA interactions

  , Nucleic Acids Research, Vol: 35, Pages: 1-8, ISSN: 0305-1048

  The established protocol for DNase I footprinting has been modified to allow multiple parallel reactions to be rapidly performed in 96-well microtitre plates. By scrutinizing every aspect of the traditional method and making appropriate modifications it has been possible to considerably reduce the time, risk of sample loss and complexity of footprinting, whilst dramatically increasing the yield of data (30-fold). A semi-automated analysis system has also been developed to present footprinting data as an estimate of the binding affinity of each tested compound to any base pair in the assessed DNA sequence, giving an intuitive ‘one compound–one line’ scheme. Here, we demonstrate the screening capabilities of the 96-well assay and the subsequent data analysis using a series of six pyrrolobenzodiazepine-polypyrrole compounds and human Topoisomerase II alpha promoter DNA. The dramatic increase in throughput, quantified data and decreased handling time allow, for the first time, DNase I footprinting to be used as a screening tool to assess DNA-binding agents.

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• Journal article
  Desai SR, 2007,

  Unsuspected pulmonary embolism on CT scanning: yet another headache for clinicians?

  , THORAX, Vol: 62, Pages: 470-472, ISSN: 0040-6376
  • Author Web Link
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  • Citations: 34
• Journal article
  Penas MM, Hervas-Aguilar A, Munera-Huertas T, Reoyo E, Penalva MA, Arst HN, Tilburn Jet al., 2007,

  Further characterization of the signaling proteolysis step in the <i>Aspergillus nidulans</i> pH signal transduction pathway

  , EUKARYOTIC CELL, Vol: 6, Pages: 960-970, ISSN: 1535-9778
  • Author Web Link
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  • Citations: 53
• Journal article
  Feary J, Britton J, 2007,

  Dietary supplements and asthma: another one bites the dust

  , THORAX, Vol: 62, Pages: 466-468, ISSN: 0040-6376
  • Author Web Link
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  • Citations: 13
• Journal article
  Marwick JA, Ito K, Adcock IM, Kirkham PAet al., 2007,

  Oxidative stress and steroid resistance in asthma and COPD: pharmacological manipulation of HDAC-2 as a therapeutic strategy

  , EXPERT OPINION ON THERAPEUTIC TARGETS, Vol: 11, Pages: 745-755, ISSN: 1472-8222
  • Author Web Link
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  • Citations: 54
• Journal article
  Bozkurt O, Hakki EE, Akkaya MS, 2007,

  Isolation and sequence analysis of wheat NBS-LRR type disease resistance gene analogs using degenerate PCR primers

  , BIOCHEMICAL GENETICS, Vol: 45, Pages: 469-486, ISSN: 0006-2928
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  • Citations: 13
• Journal article
  Regamey N, Hilliard TN, Saglani S, Zhu J, Scallan M, Balfour-Lynn IM, Rosenthal M, Jeffery PK, Alton EWFW, Bush A, Davies JCet al., 2007,

  Quality, size, and composition of pediatric endobronchial biopsies in cystic fibrosis

  , CHEST, Vol: 131, Pages: 1710-1717, ISSN: 0012-3692
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  • Citations: 27
• Journal article
  Davies JC, Rubin BK, 2007,

  Emerging and unusual gram-negative infections in cystic fibrosis

  , SEMINARS IN RESPIRATORY AND CRITICAL CARE MEDICINE, Vol: 28, Pages: 312-321, ISSN: 1069-3424
  • Author Web Link
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  • Citations: 67
• Journal article
  Goh NSL, Veeraraghavan S, Desai SR, Cramer D, Hansell DM, Denton CP, Black CM, du Bois RM, Wells AUet al., 2007,

  Bronchoalveolar lavage cellular profiles in patients with systemic sclerosis-associated interstitial lung disease are not predictive of disease progression

  , ARTHRITIS AND RHEUMATISM, Vol: 56, Pages: 2005-2012, ISSN: 0004-3591
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  • Citations: 120
• Journal article
  Van Vliet LD, Ellis T, Foley PJ, Liu L, Pfeffer FM, Russell RA, Warrener RN, Hollfelder F, Waring MJet al., 2007,

  Molecular recognition of DNA by rigid [<i>n</i>]-polynorbornane-derived bifunctional intercalators:: Synthesis and evaluation of their binding properties

  , JOURNAL OF MEDICINAL CHEMISTRY, Vol: 50, Pages: 2326-2340, ISSN: 0022-2623
  • Author Web Link
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  • Citations: 39

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