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  • Journal article
    Tanzer MM, Arst HN, Skalchunes AR, Coffin M, Darveaux BA, Heiniger RW, Shuster JRet al., 2003,

    Global nutritional profiling for mutant and chemical mode-of-action analysis in filamentous fungi.

    , Funct Integr Genomics, Vol: 3, Pages: 160-170, ISSN: 1438-793X

    We describe a method for gene function discovery and chemical mode-of-action analysis via nutrient utilization using a high throughput Nutritional Profiling platform suitable for filamentous microorganisms. We have optimized the growth conditions for each fungal species to produce reproducible optical density growth measurements in microtiter plates. We validated the Nutritional Profiling platform using a nitrogen source utilization assay to analyze 21 Aspergillus nidulans strains with mutations in the master nitrogen regulatory gene, areA. Analysis of these data accurately reproduced expected results and provided new data to demonstrate that this platform is suitable for fine level phenotyping of filamentous fungi. Next, we analyzed the differential responses of two fungal species to a glutamine synthetase inhibitor, illustrating chemical mode-of-action analysis. Finally, a comparative phenotypic study was performed to characterize carbon catabolite repression in four fungal species using a carbon source utilization assay. The results demonstrate differentiation between two Aspergillus species and two diverse plant pathogens and provide a wealth of new data on fungal nutrient utilization. Thus, these assays can be used for gene function and chemical mode-of-action analysis at the whole organism level as well as interspecies comparisons in a variety of filamentous fungi. Additionally, because uniform distribution of growth within wells is maintained, comparisons between yeast and filamentous forms of a single organism can be performed.

  • Journal article
    Ranganathan SC, Goetz I, Hoo AF, Lum S, Castle R, Stocks Jet al., 2003,

    Assessment of tidal breathing parameters in infants with cystic fibrosis

    , EUROPEAN RESPIRATORY JOURNAL, Vol: 22, Pages: 761-766, ISSN: 0903-1936
  • Journal article
    Johnson H, Whiteford JR, Eckert SE, Spanu PDet al., 2003,

    Production and secretion of <i>Aspergillus nidulans</i> catalase B in filamentous fungi driven by the promoter and signal peptide of the <i>Cladosporium fulvum</i> hydrophobin gene <i>hcf-1</i>

    , CURRENT GENETICS, Vol: 44, Pages: 155-163, ISSN: 0172-8083
  • Journal article
    Allen M, Heinzmann A, Noguchi E, Abecasis G, Broxholme J, Ponting CP, Bhattacharyya S, Tinsley J, Zhang YM, Holt R, Jones EY, Lench N, Carey A, Jones H, Dickens NJ, Dimon C, Nicholls R, Baker C, Xue LZ, Townsend E, Kabesch M, Weiland SK, Carr D, von Mutius E, Adcock IM, Barnes PJ, Lathrop GM, Edwards M, Moffatt MF, Cookson WOCMet al., 2003,

    Positional cloning of a novel gene influencing asthma from Chromosome 2q14

    , NATURE GENETICS, Vol: 35, Pages: 258-263, ISSN: 1061-4036
  • Journal article
    Traherne JA, Hill MR, D'Amato M, Broxholme J, Mott R, Moffatt MF, Cookson WOCMet al., 2003,

    LD mapping of maternally and non-maternally derived alleles and atopy in <i>FcεRI-</i>β

    , HUMAN MOLECULAR GENETICS, Vol: 12, Pages: 2577-2585, ISSN: 0964-6906
  • Journal article
    Solomon PS, Thomas SW, Spanu P, Oliver RPet al., 2003,

    The utilisation of di/tripeptides by <i>Stagonospora nodorum</i> is dispensable for wheat infection

    , PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, Vol: 63, Pages: 191-199, ISSN: 0885-5765
  • Journal article
    Arst HN, Peñalva MA, 2003,

    Recognizing gene regulation by ambient pH

    , FUNGAL GENETICS AND BIOLOGY, Vol: 40, Pages: 1-3, ISSN: 1087-1845
  • Journal article
    Davies J, Payne D, 2003,

    Research applications of bronchoscopy.

    , Paediatr Respir Rev, Vol: 4, Pages: 230-236, ISSN: 1526-0542

    The technique of flexible bronchoscopy creates unique research opportunities to investigate the underlying mechanisms of respiratory disease in children, by sampling directly from the airway. Bronchoscopy is, however, invasive and the ethics of performing this type of procedure for research purposes must be considered carefully at the outset. This article discusses the research applications of bronchoscopy in children, focusing on the ethical and safety issues, the types of procedure that can be performed and their application to the investigation of specific diseases. Acceptance of the use of bronchoscopy as a research tool is increasing. Future work should focus on the comparison of invasive and non-invasive techniques, the use of bronchoscopy in longitudinal and interventional studies and the development of new, highly sensitive techniques to allow us to study disease pathogenesis in greater detail. Collaboration between centres should be encouraged to maximise the use of samples obtained.

  • Journal article
    Arst HN, Nicholas HB, Caddick MX, 2003,

    Re:: Watts et al. proteins 2002;48:161-168

    , PROTEINS-STRUCTURE FUNCTION AND GENETICS, Vol: 52, Pages: 125-128, ISSN: 0887-3585
  • Journal article
    Desai SR, Wells AU, Rubens MB, du Bois RM, Hansell DMet al., 2003,

    Traction bronchiectasis in cryptogenic fibrosing alveolitis: associated computed tomographic features and physiological significance

    , EUROPEAN RADIOLOGY, Vol: 13, Pages: 1801-1808, ISSN: 0938-7994

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