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Journal articleMorar N, Cookson WOCM, Harper JI, et al., 2007,
Filaggrin mutations in children with severe atopic dermatitis
, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 127, Pages: 1667-1672, ISSN: 0022-202X- Author Web Link
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- Citations: 153
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Journal articleGutmann DAP, Mizohata E, Newstead S, et al., 2007,
A high-throughput method for membrane protein solubility screening: The ultracentrifugation dispersity sedimentation assay
, PROTEIN SCIENCE, Vol: 16, Pages: 1422-1428, ISSN: 0961-8368- Author Web Link
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- Citations: 52
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Journal articleBush A, Davies J, 2007,
Response to mallory: You are civilized, but still wrong, Dr. Mallory
, PEDIATRIC PULMONOLOGY, Vol: 42, Pages: 658-658, ISSN: 8755-6863- Author Web Link
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- Citations: 2
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Journal articleEllis T, Evans DA, Martin CRH, et al., 2007,
A 96-well DNase I footprinting screen for drug-DNA interactions
, Nucleic Acids Research, Vol: 35, Pages: 1-8, ISSN: 0305-1048The established protocol for DNase I footprinting has been modified to allow multiple parallel reactions to be rapidly performed in 96-well microtitre plates. By scrutinizing every aspect of the traditional method and making appropriate modifications it has been possible to considerably reduce the time, risk of sample loss and complexity of footprinting, whilst dramatically increasing the yield of data (30-fold). A semi-automated analysis system has also been developed to present footprinting data as an estimate of the binding affinity of each tested compound to any base pair in the assessed DNA sequence, giving an intuitive ‘one compound–one line’ scheme. Here, we demonstrate the screening capabilities of the 96-well assay and the subsequent data analysis using a series of six pyrrolobenzodiazepine-polypyrrole compounds and human Topoisomerase II alpha promoter DNA. The dramatic increase in throughput, quantified data and decreased handling time allow, for the first time, DNase I footprinting to be used as a screening tool to assess DNA-binding agents.
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Journal articleDesai SR, 2007,
Unsuspected pulmonary embolism on CT scanning: yet another headache for clinicians?
, THORAX, Vol: 62, Pages: 470-472, ISSN: 0040-6376- Author Web Link
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- Citations: 34
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Journal articlePenas MM, Hervas-Aguilar A, Munera-Huertas T, et al., 2007,
Further characterization of the signaling proteolysis step in the <i>Aspergillus nidulans</i> pH signal transduction pathway
, EUKARYOTIC CELL, Vol: 6, Pages: 960-970, ISSN: 1535-9778- Author Web Link
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- Citations: 53
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Journal articleFeary J, Britton J, 2007,
Dietary supplements and asthma: another one bites the dust
, THORAX, Vol: 62, Pages: 466-468, ISSN: 0040-6376- Author Web Link
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- Citations: 13
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Journal articleMarwick JA, Ito K, Adcock IM, et al., 2007,
Oxidative stress and steroid resistance in asthma and COPD: pharmacological manipulation of HDAC-2 as a therapeutic strategy
, EXPERT OPINION ON THERAPEUTIC TARGETS, Vol: 11, Pages: 745-755, ISSN: 1472-8222- Author Web Link
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- Citations: 54
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Journal articleBozkurt O, Hakki EE, Akkaya MS, 2007,
Isolation and sequence analysis of wheat NBS-LRR type disease resistance gene analogs using degenerate PCR primers
, BIOCHEMICAL GENETICS, Vol: 45, Pages: 469-486, ISSN: 0006-2928- Author Web Link
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- Citations: 13
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Journal articleRegamey N, Hilliard TN, Saglani S, et al., 2007,
Quality, size, and composition of pediatric endobronchial biopsies in cystic fibrosis
, CHEST, Vol: 131, Pages: 1710-1717, ISSN: 0012-3692- Author Web Link
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- Citations: 27
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