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  • Journal article
    Leung J, Karachaliou M, Alves C, Diallinas G, Byrne Bet al., 2010,

    Expression and purification of a functional uric acid-xanthine transporter (UapA)

    , PROTEIN EXPRESSION AND PURIFICATION, Vol: 72, Pages: 139-146, ISSN: 1046-5928
  • Journal article
    Hervás-Aguilar A, Rodríguez-Galán O, Galindo A, Abenza JF, Arst HN, Peñalva MAet al., 2010,

    Characterization of Aspergillus nidulans DidB Did2, a non-essential component of the multivesicular body pathway.

    , Fungal Genet Biol, Vol: 47, Pages: 636-646, ISSN: 1087-1845

    ESCRT-III heteropolymers mediate membrane protein cargo sorting into multivesicular endosomes for subsequent vacuolar degradation. We studied the localization of largely uncharacterized Aspergillus nidulans ESCRT-III using its key structural component Vps32 and the 'associated' component DidB(Did2). Vps32-GFP localizes to motile early endosomes as reported, but predominates in aggregates often associated with vacuoles due to inability to dissociate from endosomes. DidB(Did)(2) regulating Vps4 (the ATPase disassembling ESCRT-III) is not essential. Consistent with this accessory role, didB Delta is unable to block the MVB sorting of the glutamate transporter AgtA, but increases its steady-state level and mislocalizes a fraction of the permease to the plasma membrane under conditions promoting its vacuolar targeting. didB Delta exacerbates the dominant-negative growth defect resulting from Vps32-GFP over-expression. A proportion of DidB-GFP is detectable in early endosomes colocalizing with RabA(Rab5) and accumulating in nudA1 tips, suggesting that ESCRT-III assembles on endosomes from the early steps of the endocytic pathway.
  • Journal article
    Findon H, Calcagno-Pizarelli AM, Martínez JL, Spielvogel A, Markina-Iñarrairaegui A, Indrakumar T, Ramos J, Peñalva MA, Espeso EA, Arst HNet al., 2010,

    Analysis of a novel calcium auxotrophy in Aspergillus nidulans.

    , Fungal Genet Biol, Vol: 47, Pages: 647-655, ISSN: 1096-0937

    In Aspergillus nidulans a combination of null mutations in halA, encoding a protein kinase, and sltA, encoding a zinc-finger transcription factor having no yeast homologues, results in an elevated calcium requirement ('calcium auxotrophy') without impairing net calcium uptake. sltA(-) (+/-halA(-)) mutations result in hypertrophy of the vacuolar system. In halA(-)sltA(-) (and sltA(-)) strains, transcript levels for pmcA and pmcB, encoding vacuolar Ca(2+)-ATPase homologues, are highly elevated, suggesting a regulatory relationship between vacuolar membrane area and certain vacuolar membrane ATPase levels. Deletion of both pmcA and pmcB strongly suppresses the 'calcium auxotrophy'. Therefore the 'calcium auxotrophy' possibly results from excessive vacuolar calcium sequestration, causing cytosolic calcium deprivation. Null mutations in nhaA, homologous to Saccharomyces cerevisiae NHA1, encoding a plasma membrane Na(+)/H(+) antiporter effluxing Na(+) and K(+), and a non-null mutation in trkB, homologous to S. cerevisiae TRK1, encoding a plasma membrane high affinity K(+) transporter, also suppress the calcium auxotrophy.
  • Journal article
    Schmidt HJ, Bhandari V, Bhandari A, Davies J, Marshall BC, Praud J-P, Zar HJ, Rubin BKet al., 2010,

    The future in paediatric respirology

    , RESPIROLOGY, Vol: 15, Pages: 733-741, ISSN: 1323-7799
  • Journal article
    Chrzanowski W, Abou Neel EA, Lee K-Y, Bismarck A, Young AM, Hart AD, Dalby MJ, Knowles JCet al., 2010,

    Tailoring Cell Behavior on Polymers by the Incorporation of Titanium Doped Phosphate Glass Filler

    , ADVANCED ENGINEERING MATERIALS, Vol: 12, Pages: B298-B308, ISSN: 1438-1656
  • Journal article
    Griesenbach U, Sumner-Jones SG, Holder E, Munkonge FM, Wodehouse T, Smith SN, Wasowicz MY, Pringle I, Casamayor I, Chan M, Coles R, Cornish N, Dewar A, Doherty A, Farley R, Green A-M, Jones BL, Larsen MDB, Lawton AE, Manvell M, Painter H, Singh C, Somerton L, Stevenson B, Varathalingam A, Siegel C, Scheule RK, Cheng SH, Davies JC, Porteous DJ, Gill DR, Boyd AC, Hyde SC, Alton EWFWet al., 2010,

    Limitations of the Murine Nose in the Development of Nonviral Airway Gene Transfer

    , AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, Vol: 43, Pages: 46-54, ISSN: 1044-1549
  • Journal article
    Oliva R, Win J, Raffaele S, Boutemy L, Bozkurt TO, Chaparro-Garcia A, Segretin ME, Stam R, Schornack S, Cano LM, van Damme M, Huitema E, Thines M, Banfield MJ, Kamoun Set al., 2010,

    Recent developments in effector biology of filamentous plant pathogens (vol 12, pg 705, 2010)

    , CELLULAR MICROBIOLOGY, Vol: 12, Pages: 1015-1015, ISSN: 1462-5814
  • Journal article
    Ikem VO, Menner A, Bismarck A, 2010,

    High-Porosity Macroporous Polymers Sythesized from Titania-Particle-Stabilized Medium and High Internal Phase Emulsions

    , LANGMUIR, Vol: 26, Pages: 8836-8841, ISSN: 0743-7463
  • Journal article
    Oliva R, Win J, Raffaele S, Boutemy L, Bozkurt TO, Chaparro-Garcia A, Segretin ME, Stam R, Schornack S, Cano LM, van Damme M, Huitema E, Thines M, Banfield MJ, Kamoun Set al., 2010,

    Recent developments in effector biology of filamentous plant pathogens

    , CELLULAR MICROBIOLOGY, Vol: 12, Pages: 705-715, ISSN: 1462-5814
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    • Citations: 87
  • Journal article
    Solis R, Lobos G, Walker SF, Fisher M, Bosch Jet al., 2010,

    Presence of Batrachochytrium dendrobatidis in feral populations of Xenopus laevis in Chile

    , BIOLOGICAL INVASIONS, Vol: 12, Pages: 1641-1646, ISSN: 1387-3547

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