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  • Journal article
    JONES SA, ARST HN, MACDONALD DW, 1981,

    IDENTIFICATION OF STRUCTURAL GENES IN THE PRN GENE-CLUSTER OF ASPERGILLUS-NIDULANS

    , HEREDITY, Vol: 46, Pages: 287-287, ISSN: 0018-067X
  • Journal article
    Arst HN, Scazzocchio C, 1980,

    A method for monitoring macromolecular synthesis in filamentous fungi and its use to study control of nucleic acid synthesis in Aspergillus nidulans

    , Journal of General Microbiology, Vol: 118, Pages: 443-454, ISSN: 0022-1287

    A method is described for measuring the syntheses of macromolecules in the filamentous fungus A. nidulans. It involves growth of mycelium on small filter papers in the presence of radioactively labelled L-leucine to monitor total protein synthesis, and hence growth, and following the incorporation of differently labelled D-uridine (to measure nucleic acid synthesis) or L-leucine (to measure protein synthesis) under various conditions such as starvation for a metabolite or the presence of an inhibitor. Comparison of <sup>3</sup>H/<sup>14</sup>C or <sup>14</sup>C/<sup>3</sup>H labelling ratios (depending on the labelling combination used) shows the effects of the treatments on nucleic acid or protein synthesis. The method allows large numbers of measurements to be made and enables economical use of radioisotopes. Results are presented to show that, under the labelling conditions used, uridine is incorporated mainly into stable RNA. The method is used to demonstrate probable stringent control of stable RNA synthesis in A. nidulans and that inhibitors of protein synthesis such as cycloheximide and anisomycin also inhibit stable RNA synthesis. In contrast, starvation for meso-inositol affects nucleic acid synthesis much more strongly than it affects protein synthesis.
  • Journal article
    Arst HN, MacDonald DW, Jones SA, 1980,

    Regulation of proline transport in Aspergillus nidulans

    , Journal of General Microbiology, Vol: 116, Pages: 285-294, ISSN: 0022-1287
    • Cite
    • Citations: 37
  • Journal article
    ARST HN, RAND KN, BAILEY CR, 1980,

    AN INSERTIONAL TRANSLOCATION SEPARATING THE TIGHTLY LINKED STRUCTURAL GENES FOR NITRATE AND NITRITE REDUCTASES IN ASPERGILLUS-NIDULANS

    , HEREDITY, Vol: 44, Pages: 289-289, ISSN: 0018-067X
  • Journal article
    ARST HN, BAILEY CR, 1980,

    GENETIC-EVIDENCE FOR A 2ND ASPARAGINASE IN ASPERGILLUS-NIDULANS

    , JOURNAL OF GENERAL MICROBIOLOGY, Vol: 121, Pages: 243-247, ISSN: 0022-1287
    • Cite
    • Citations: 11
  • Journal article
    ARST HN, BAILEY CR, PENFOLD HA, 1980,

    A POSSIBLE ROLE FOR ACID-PHOSPHATASE IN GAMMA-AMINO-NORMAL-BUTYRATE UPTAKE IN ASPERGILLUS-NIDULANS

    , ARCHIVES OF MICROBIOLOGY, Vol: 125, Pages: 153-158, ISSN: 0302-8933
    • Cite
    • Citations: 19
  • Journal article
    BAILEY CR, ARST HN, PENFOLD HA, 1980,

    A 3RD GENE AFFECTING GABA TRANSAMINASE LEVELS IN ASPERGILLANS-NIDULUS

    , GENETICAL RESEARCH, Vol: 36, Pages: 167-180, ISSN: 0016-6723
  • Journal article
    ARST HN, BAILEY CR, PENFOLD HA, 1980,

    A ROLE FOR ACID-PHOSPHATASE IN GABA TRANSPORT IN ASPERGILLUS-NIDULANS

    , HEREDITY, Vol: 44, Pages: 289-289, ISSN: 0018-067X
  • Journal article
    ARST HN, MACDONALD DW, JONES SA, 1980,

    REGULATION OF PROLINE TRANSPORT IN ASPERGILLUS-NIDULANS

    , JOURNAL OF GENERAL MICROBIOLOGY, Vol: 116, Pages: 285-294, ISSN: 0022-1287
  • Journal article
    ARST HN, SCAZZOCCHIO C, 1980,

    A METHOD FOR MONITORING MACROMOLECULAR-SYNTHESIS IN FILAMENTOUS FUNGI AND ITS USE TO STUDY CONTROL OF NUCLEIC-ACID SYNTHESIS IN ASPERGILLUS-NIDULANS

    , JOURNAL OF GENERAL MICROBIOLOGY, Vol: 118, Pages: 443-454, ISSN: 0022-1287

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