@article{Bhutada:2022:10.1016/j.mec.2022.e00192,
author = {Bhutada, G and Menard, G and Bhunia, RK and Hapeta, PP and Ledesma-Amaro, R and Eastmond, PJ},
doi = {10.1016/j.mec.2022.e00192},
journal = {Metabolic Engineering Communications},
pages = {1--10},
title = {Production of human milk fat substitute by engineered strains of Yarrowia lipolytica.},
url = {http://dx.doi.org/10.1016/j.mec.2022.e00192},
volume = {14},
year = {2022}
}
TY - JOUR
AB - Human milk fat has a distinctive stereoisomeric structure where palmitic acid is esterified to the middle (sn-2) position on the glycerol backbone of the triacylglycerol and unsaturated fatty acids to the outer (sn-1/3) positions. This configuration allows for more efficient nutrient absorption in the infant gut. However, the fat used in most infant formulas originates from plants, which exclude palmitic acid from the sn-2 position. Oleaginous yeasts provide an alternative source of lipids for human nutrition. However, these yeasts also exclude palmitic acid from the sn-2 position of their triacylglycerol. Here we show that Yarrowia lipolytica can be engineered to produce triacylglycerol with more than 60% of the palmitic acid in the sn-2 position, by expression of lysophosphatidic acid acyltransferases with palmitoyl-Coenzyme A specificity. The engineered Y. lipolytica strains can be cultured on glycerol, glucose, palm oil or a mixture of substrates, under nitrogen limited condition, to produce triacylglycerol with a fatty acid composition that resembles human milk fat, in terms of the major molecular species (palmitic, oleic and linoleic acids). Culture on palm oil or a mixture of glucose and palm oil produced the highest lipid titre and a triacylglycerol composition that is most similar with human milk fat. Our data show that an oleaginous yeast can be engineered to produce a human milk fat substitute (β-palmitate), that could be used as an ingredient in infant formulas.
AU - Bhutada,G
AU - Menard,G
AU - Bhunia,RK
AU - Hapeta,PP
AU - Ledesma-Amaro,R
AU - Eastmond,PJ
DO - 10.1016/j.mec.2022.e00192
EP - 10
PY - 2022///
SN - 2214-0301
SP - 1
TI - Production of human milk fat substitute by engineered strains of Yarrowia lipolytica.
T2 - Metabolic Engineering Communications
UR - http://dx.doi.org/10.1016/j.mec.2022.e00192
UR - https://www.ncbi.nlm.nih.gov/pubmed/35036316
UR - https://www.sciencedirect.com/science/article/pii/S2214030122000013?via%3Dihub
UR - http://hdl.handle.net/10044/1/93997
VL - 14
ER -