@article{Wang:2022:10.1021/acssynbio.1c00613,
author = {Wang, K and Shi, T-Q and Wang, J and Wei, P and Ledesma-Amaro, R and Ji, X-J},
doi = {10.1021/acssynbio.1c00613},
journal = {ACS Synthetic Biology},
pages = {1542--1554},
title = {Engineering the lipid and fatty acid metabolism in Yarrowia lipolytica for sustainable production of high oleic oils},
url = {http://dx.doi.org/10.1021/acssynbio.1c00613},
volume = {11},
year = {2022}
}

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TY  - JOUR
AB  - Oleic acid is widely applied in the chemical, material, nutritional, and pharmaceutical industries. However, the current production of oleic acid via high oleic plant oils is limited by the long growth cycle and climatic constraints. Moreover, the global demand for high oleic plant oils, especially the palm oil, has emerged as the driver of tropical deforestation causing tropical rainforest destruction, climate change, and biodiversity loss. In the present study, an alternative and sustainable strategy for high oleic oil production was established by reprogramming the metabolism of the oleaginous yeast Yarrowia lipolytica using a two-layer "push-pull-block" strategy. Specifically, the fatty acid synthesis pathway was first engineered to increase oleic acid proportion by altering the fatty acid profiles. Then, the content of storage oils containing oleic acid was boosted by engineering the synthesis and degradation pathways of triacylglycerides. The strain resulting from this two-layer engineering strategy produced the highest titer of high oleic microbial oil reaching 56 g/L with 84% oleic acid in fed-batch fermentation, representing a remarkable improvement of a 110-fold oil titer and 2.24-fold oleic acid proportion compared with the starting strain. This alternative and sustainable method for high oleic oil production shows the potential of substitute planting.
AU  - Wang,K
AU  - Shi,T-Q
AU  - Wang,J
AU  - Wei,P
AU  - Ledesma-Amaro,R
AU  - Ji,X-J
DO  - 10.1021/acssynbio.1c00613
EP  - 1554
PY  - 2022///
SN  - 2161-5063
SP  - 1542
TI  - Engineering the lipid and fatty acid metabolism in Yarrowia lipolytica for sustainable production of high oleic oils
T2  - ACS Synthetic Biology
UR  - http://dx.doi.org/10.1021/acssynbio.1c00613
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35311250
UR  - https://pubs.acs.org/doi/10.1021/acssynbio.1c00613
UR  - http://hdl.handle.net/10044/1/96228
VL  - 11
ER  - 

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