Citation

BibTex format

@article{Sun:2022:10.1007/s00253-022-11918-x,
author = {Sun, M and Gao, AX and Ledesma-Amaro, R and Li, A and Wang, R and Nie, J and Zheng, P and Yang, Y and Bai, Z and Liu, X},
doi = {10.1007/s00253-022-11918-x},
journal = {Applied Microbiology and Biotechnology},
pages = {2953--2967},
title = {Hypersecretion of OmlA antigen in Corynebacterium glutamicum through high-throughput based development process},
url = {http://dx.doi.org/10.1007/s00253-022-11918-x},
volume = {106},
year = {2022}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Outer membrane lipoprotein A (OmlA) is a vaccine antigen against porcine contagious pleuropneumonia (PCP), a disease severely affecting the swine industry. Here, we aimed to systematically potentiate the secretory production of OmlA in Corynebacterium glutamicum (C. glutamicum), a widely used microorganism in the food industry, by establishing a holistic development process based on our high-throughput culture platform. The expression patterns, expression element combinations, medium composition, and induction conditions were comprehensively screened or optimized in microwell plates (MWPs), followed by fermentation parameter optimization in a 4 × 1 L parallel fermentation system (CUBER4). An unprecedented yield of 1.01 g/L OmlA was ultimately achieved in a 5-L bioreactor following the scaling-up strategy of fixed oxygen mass transfer coefficient (kLa), and the produced OmlA antigen showed well-protective immunity against Actinobacillus pleuropneumoniae challenge. This result provides a rapid and reliable pipeline to achieve the hyper-production of OmlA, and possibly other recombinant vaccines, in C. glutamicum.Key Points• Established a holistic development process and applied it to potentiate the secretion of OmlA.• The secretion of OmlA reached an unprecedented yield of 1.01 g/L.• The recombinant OmlA antigen induced efficient protective immunity.
AU - Sun,M
AU - Gao,AX
AU - Ledesma-Amaro,R
AU - Li,A
AU - Wang,R
AU - Nie,J
AU - Zheng,P
AU - Yang,Y
AU - Bai,Z
AU - Liu,X
DO - 10.1007/s00253-022-11918-x
EP - 2967
PY - 2022///
SN - 0175-7598
SP - 2953
TI - Hypersecretion of OmlA antigen in Corynebacterium glutamicum through high-throughput based development process
T2 - Applied Microbiology and Biotechnology
UR - http://dx.doi.org/10.1007/s00253-022-11918-x
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000784385900005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/96864
VL - 106
ER -