Citation

BibTex format

@article{Lee:2022:10.1039/d2sc00539e,
author = {Lee, HJ and Ehsan, M and Zhang, X and Katsube, S and Munk, CF and Wang, H and Ahmed, W and Kumar, A and Byrne, B and Loland, CJ and Guan, L and Liu, X and Chae, PS},
doi = {10.1039/d2sc00539e},
journal = {Chemical Science},
pages = {5750--5759},
title = {Development of 1,3-acetonedicarboxylate-derived glucoside amphiphiles (ACAs) for membrane protein study},
url = {http://dx.doi.org/10.1039/d2sc00539e},
volume = {13},
year = {2022}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Detergents are extensively used for membrane protein manipulation. Membrane proteins solubilized in conventional detergents are prone to denaturation and aggregation, rendering downstream characterization of these bio-macromolecules difficult. Although many amphiphiles have been developed to overcome the limited efficacy of conventional detergents for protein stabilization, only a handful of novel detergents have so far proved useful for membrane protein structural studies. Here, we introduce 1,3-acetonedicarboxylate-derived amphiphiles (ACAs) containing three glucose units and two alkyl chains as head and tail groups, respectively. The ACAs incorporate two different patterns of alkyl chain attachment to the core detergent unit, generating two sets of amphiphiles: ACA-As (asymmetrically alkylated) and ACA-Ss (symmetrically alkylated). The difference in the attachment pattern of the detergent alkyl chains resulted in minor variation in detergent properties such as micelle size, critical micelle concentration, and detergent behaviors toward membrane protein extraction and stabilization. In contrast, the impact of the detergent alkyl chain length on protein stability was marked. The two C11 variants (ACA-AC11 and ACA-SC11) were most effective at stabilizing the tested membrane proteins. The current study not only introduces new glucosides as tools for membrane protein study, but also provides detergent structure–property relationships important for future design of novel amphiphiles.
AU - Lee,HJ
AU - Ehsan,M
AU - Zhang,X
AU - Katsube,S
AU - Munk,CF
AU - Wang,H
AU - Ahmed,W
AU - Kumar,A
AU - Byrne,B
AU - Loland,CJ
AU - Guan,L
AU - Liu,X
AU - Chae,PS
DO - 10.1039/d2sc00539e
EP - 5759
PY - 2022///
SN - 2041-6520
SP - 5750
TI - Development of 1,3-acetonedicarboxylate-derived glucoside amphiphiles (ACAs) for membrane protein study
T2 - Chemical Science
UR - http://dx.doi.org/10.1039/d2sc00539e
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000787743400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://pubs.rsc.org/en/content/articlelanding/2022/SC/D2SC00539E
UR - http://hdl.handle.net/10044/1/100304
VL - 13
ER -