Citation

BibTex format

@article{Xu:2022:10.1038/s41467-022-30826-2,
author = {Xu, Y and Wang, X and Zhang, C and Zhou, X and Xu, X and Han, L and Lv, X and Liu, Y and Liu, S and Li, J and Du, G and Chen, J and Ledesma-Amaro, R and Liu, L},
doi = {10.1038/s41467-022-30826-2},
journal = {Nature Communications},
title = {De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts},
url = {http://dx.doi.org/10.1038/s41467-022-30826-2},
volume = {13},
year = {2022}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - High-sugar diet causes health problems, many of which can be addressed with the use of sugar substitutes. Rubusoside and rebaudiosides are interesting molecules, considered the next generation of sugar substitutes due to their low-calorie, superior sweetness and organoleptic properties. However, their low abundance in nature makes the traditional plant extraction process neither economical nor environmental-friendly. Here we engineer baker’s yeast Saccharomyces cerevisiae as a chassis for the de novo production of rubusoside and rebaudiosides. In this process, we identify multiple issues that limit the production, including rate-liming steps, product stress on cellular fitness and unbalanced metabolic networks. We carry out a systematic engineering strategy to solve these issues, which produces rubusoside and rebaudiosides at titers of 1368.6 mg/L and 132.7 mg/L, respectively. The rubusoside chassis strain here constructed paves the way towards a sustainable, large-scale fermentation-based manufacturing of diverse rebaudiosides.
AU - Xu,Y
AU - Wang,X
AU - Zhang,C
AU - Zhou,X
AU - Xu,X
AU - Han,L
AU - Lv,X
AU - Liu,Y
AU - Liu,S
AU - Li,J
AU - Du,G
AU - Chen,J
AU - Ledesma-Amaro,R
AU - Liu,L
DO - 10.1038/s41467-022-30826-2
PY - 2022///
SN - 2041-1723
TI - De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-022-30826-2
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000804921800024&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://www.nature.com/articles/s41467-022-30826-2
UR - http://hdl.handle.net/10044/1/98860
VL - 13
ER -