BibTex format

author = {Ledesma, Amaro R and Ellis, T and Shaw, W and Studena, L and Roy, K and Mccarty, N and Graham, A and Hapeta, P},
journal = {Nature Communications},
title = {Inducible expression of large gRNA arrays for multiplexed CRISPRai applications},
url = {},
volume = {13},
year = {2022}

RIS format (EndNote, RefMan)

AB - CRISPR gene activation and inhibition (CRISPRai) has become a powerful synthetic tool for influencing the expression of native genes for foundational studies, cellular reprograming, and metabolic engineering. Here we develop a method for near leak-free, inducible expression of a polycistronic array containing up to 24 gRNAs from two orthogonal CRISPR/Cas systems to increase CRISPRai multiplexing capacity and target gene flexibility. To achieve strong inducibility, we created a technology to silence gRNA expression within the array in the absence of the inducer, since we found that long gRNA arrays for CRISPRai can express themselves even without promoter. Using this method, we create a highly tuned and easy-to-use CRISPRai toolkit in the industrially relevant yeast, Saccharomyces cerevisiae, establishing the first system to combine simultaneous activation and repression, large multiplexing capacity, and inducibility. We demonstrate this platform by targeting 11 genes in central metabolism in a single transformation, achieving a 45-fold increase in succinic acid, which could be precisely controlled in an inducible manner. Our method offers a highly effective way to regulate genes and rewire metabolism in yeast, with principles of gRNA array construction and inducibility that should extend to other chassis organisms.
AU - Ledesma,Amaro R
AU - Ellis,T
AU - Shaw,W
AU - Studena,L
AU - Roy,K
AU - Mccarty,N
AU - Graham,A
AU - Hapeta,P
PY - 2022///
SN - 2041-1723
TI - Inducible expression of large gRNA arrays for multiplexed CRISPRai applications
T2 - Nature Communications
UR -
VL - 13
ER -