@article{Walls:2023:10.1016/j.biortech.2022.128216,
author = {Walls, LE and Otoupal, P and Ledesma-Amaro, R and Velasquez-Orta, SB and Gladden, JM and Rios-Solis, L},
doi = {10.1016/j.biortech.2022.128216},
journal = {Bioresource Technology},
title = {Bioconversion of cellulose into bisabolene using Ruminococcus flavefaciens and Rhodosporidium toruloides},
url = {http://dx.doi.org/10.1016/j.biortech.2022.128216},
volume = {368},
year = {2023}
}

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TY  - JOUR
AB  - In this study, organic acids were demonstrated as a promising carbon source for bisabolene production by the non-conventional yeast, Rhodosporidium toruloides, at microscale with a maximum titre of 1055 ± 7 mg/L. A 125-fold scale-up of the optimal process, enhanced bisabolene titres 2.5-fold to 2606 mg/L. Implementation of a pH controlled organic acid feeding strategy at this scale lead to a further threefold improvement in bisabolene titre to 7758 mg/L, the highest reported microbial titre. Finally, a proof-of-concept sequential bioreactor approach was investigated. Firstly, the cellulolytic bacterium Ruminococcus flavefaciens was employed to ferment cellulose, yielding 4.2 g/L of organic acids. R. toruloides was subsequently cultivated in the resulting supernatant, producing 318 ± 22 mg/L of bisabolene. This highlights the feasibility of a sequential bioprocess for the bioconversion of cellulose, into biojet fuel candidates. Future work will focus on enhancing organic acid yields and the use of real lignocellulosic feedstocks to further enhance bisabolene production.
AU  - Walls,LE
AU  - Otoupal,P
AU  - Ledesma-Amaro,R
AU  - Velasquez-Orta,SB
AU  - Gladden,JM
AU  - Rios-Solis,L
DO  - 10.1016/j.biortech.2022.128216
PY  - 2023///
SN  - 0960-8524
TI  - Bioconversion of cellulose into bisabolene using Ruminococcus flavefaciens and Rhodosporidium toruloides
T2  - Bioresource Technology
UR  - http://dx.doi.org/10.1016/j.biortech.2022.128216
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36347482
UR  - http://hdl.handle.net/10044/1/101411
VL  - 368
ER  - 

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