@article{Ghani:2023:10.1021/acs.bioconjchem.3c00042,
author = {Ghani, L and Zhang, X and Munk, CF and Hariharan, P and Lan, B and Yun, HS and Byrne, B and Guan, L and Loland, CJ and Liu, X and Chae, PS},
doi = {10.1021/acs.bioconjchem.3c00042},
journal = {Bioconjugate Chemistry},
pages = {739--747},
title = {Tris(hydroxymethyl)aminomethane linker-bearing triazine-based triglucosides for solubilization and stabilization of membrane proteins},
url = {http://dx.doi.org/10.1021/acs.bioconjchem.3c00042},
volume = {34},
year = {2023}
}

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TY  - JOUR
AB  - High-resolution membrane protein structures are essential for a fundamental understanding of the molecular basis of diverse cellular processes and for drug discovery. Detergents are widely used to extract membrane-spanning proteins from membranes and maintain them in a functional state for downstream characterization. Due to limited long-term stability of membrane proteins encapsulated in conventional detergents, development of novel agents is required to facilitate membrane protein structural study. In the current study, we designed and synthesized tris(hydroxymethyl)aminomethane linker-bearing triazine-based triglucosides (TTGs) for solubilization and stabilization of membrane proteins. When these glucoside detergents were evaluated for four membrane proteins including two G protein-coupled receptors, a few TTGs including TTG-C10 and TTG-C11 displayed markedly enhanced behaviors toward membrane protein stability relative to two maltoside detergents [DDM (n-dodecyl-β-d-maltoside) and LMNG (lauryl maltose neopentyl glycol)]. This is a notable feature of the TTGs as glucoside detergents tend to be inferior to maltoside detergents at stabilizing membrane proteins. The favorable behavior of the TTGs for membrane protein stability is likely due to the high hydrophobicity of the lipophilic groups, an optimal range of hydrophilic–lipophilic balance, and the absence of cis–trans isomerism.
AU  - Ghani,L
AU  - Zhang,X
AU  - Munk,CF
AU  - Hariharan,P
AU  - Lan,B
AU  - Yun,HS
AU  - Byrne,B
AU  - Guan,L
AU  - Loland,CJ
AU  - Liu,X
AU  - Chae,PS
DO  - 10.1021/acs.bioconjchem.3c00042
EP  - 747
PY  - 2023///
SN  - 1043-1802
SP  - 739
TI  - Tris(hydroxymethyl)aminomethane linker-bearing triazine-based triglucosides for solubilization and stabilization of membrane proteins
T2  - Bioconjugate Chemistry
UR  - http://dx.doi.org/10.1021/acs.bioconjchem.3c00042
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000973026200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://pubs.acs.org/doi/10.1021/acs.bioconjchem.3c00042
UR  - http://hdl.handle.net/10044/1/109741
VL  - 34
ER  - 

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