@article{Suwan:2019:10.1073/pnas.1906653116,
author = {Suwan, K and Waramit, S and Przystal, J and Stoneham, C and Bentayebi, K and Asavarut, P and Chongchai, A and Pothachareon, P and Lee, K-Y and Topanurak, S and Smith, T and Gelovani, J and Sidman, R and Pasqualini, R and Arap, W and Hajitou, A},
doi = {10.1073/pnas.1906653116},
journal = {Proceedings of the National Academy of Sciences of USA},
pages = {18571--18577},
title = {Next-generation of targeted AAVP vectors for systemic transgene delivery against cancer},
url = {http://dx.doi.org/10.1073/pnas.1906653116},
volume = {116},
year = {2019}
}

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TY  - JOUR
AB  - Bacteriophage (phage) have attractive advantages as delivery sys-tems compared to mammalian viruses, but have been consideredpoor  vectors  because  they  lack  evolved  strategies  to  confrontand  overcome  mammalian  cell  barriers  to  infective  agents.  Wereasoned  that  improved  efficacy  of  delivery  might  be  achievedthrough structural modification of the viral capsid to avoid pre-and post-internalization barriers to mammalian cell transduction.We generated multifunctional hybrid AAV/phage (AAVP) particlesto enable simultaneous display of targeting ligands on the phage’sminor pIII proteins and also degradation-resistance motifs on thevery numerous pVIII coat proteins. This genetic strategy of directedevolution, bestows a next-generation of AAVP particles that fea-ture resistance to fibrinogen adsorption or neutralizing antibodies,and ability to escape endolysosomal degradation. This results insuperior  gene  transfer  efficacyin  vitroand  also  in  preclinicalmouse models of rodent and human solid tumors. Thus, the uniquefunctions of our next-generation AAVP particles enable improvedtargeted gene delivery to tumor cells.
AU  - Suwan,K
AU  - Waramit,S
AU  - Przystal,J
AU  - Stoneham,C
AU  - Bentayebi,K
AU  - Asavarut,P
AU  - Chongchai,A
AU  - Pothachareon,P
AU  - Lee,K-Y
AU  - Topanurak,S
AU  - Smith,T
AU  - Gelovani,J
AU  - Sidman,R
AU  - Pasqualini,R
AU  - Arap,W
AU  - Hajitou,A
DO  - 10.1073/pnas.1906653116
EP  - 18577
PY  - 2019///
SN  - 0027-8424
SP  - 18571
TI  - Next-generation of targeted AAVP vectors for systemic transgene delivery against cancer
T2  - Proceedings of the National Academy of Sciences of USA
UR  - http://dx.doi.org/10.1073/pnas.1906653116
UR  - http://hdl.handle.net/10044/1/71885
VL  - 116
ER  - 

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