The Centre has a long history of developing new techniques for medical imaging (particularly in magnetic resonance imaging), transforming them from a primarily diagnostic modality into an interventional and therapeutic platform. This is facilitated by the Centre's strong engineering background in practical imaging and image analysis platform development, as well as advances in minimal access and robotic assisted surgery. Hamlyn has a strong tradition in pursuing basic sciences and theoretical research, with a clear focus on clinical translation.

In response to the current paradigm shift and clinical demand in bringing cellular and molecular imaging modalities to an in vivo – in situ setting during surgical intervention, our recent research has also been focussed on novel biophotonics platforms that can be used for real-time tissue characterisation, functional assessment, and intraoperative guidance during minimally invasive surgery. This includes, for example, SMART confocal laser endomicroscopy, time-resolved fluorescence spectroscopy and flexible FLIM catheters.


BibTex format

author = {Vyas, K and hughes, M and leff, DANIEL and yang, GUANG},
doi = {10.1117/1.JBO.22.2.020501},
journal = {Journal of Biomedical Optics},
title = {Methylene-blue aided rapid confocal laser endomicroscopy of breast cancer},
url = {},
volume = {22},
year = {2017}

RIS format (EndNote, RefMan)

AB - Breast conserving surgery allows complete tumor resection while maintaining acceptable cosmesis for patients. Safe and rapid intraoperative margin assessment during the procedure is important to establish the completeness of tumor excision and minimizes the need for reoperation. Confocal laser endomicroscopy has demonstrated promise for real-time intraoperative margin assessment using acriflavine staining, but it is not approved for routine in-human use. We describe a custom high-speed line-scan confocal laser endomicroscopy (LS-CLE) system at 660 nm that enables high-resolution histomorphological imaging of breast tissue stained with methylene-blue, an alternative fluorescent stain for localizing sentinel nodes during breast surgery. Preliminary imaging results on freshly excised human breast tissue specimens are presented, demonstrating the potential of methylene-blue aided rapid LS-CLE to determine the oncological status of surgical margins in-vivo.
AU - Vyas,K
AU - hughes,M
AU - leff,DANIEL
AU - yang,GUANG
DO - 10.1117/1.JBO.22.2.020501
PY - 2017///
SN - 1083-3668
TI - Methylene-blue aided rapid confocal laser endomicroscopy of breast cancer
T2 - Journal of Biomedical Optics
UR -
UR -
VL - 22
ER -