@article{Glyde:2018:10.1016/j.molcel.2018.05.021,
author = {Glyde, R and Ye, F and Jovanovic, M and Kotta-Loizou, I and Buck, M and Zhang, X},
doi = {10.1016/j.molcel.2018.05.021},
journal = {Molecular Cell},
pages = {1111--1120.e3},
title = {Structures of bacterial RNA polymerase complexes reveal mechanisms of DNA loading and transcription initiation},
url = {http://dx.doi.org/10.1016/j.molcel.2018.05.021},
volume = {70},
year = {2018}
}

Download

TY  - JOUR
AB  - Gene transcription is carried out by multi-subunit RNA polymerases (RNAP).Transcription initiation is a dynamic multi-step process that involves the opening of the double stranded DNA to form a transcription bubble and delivery of the template strand deep into the RNAP for RNA synthesis. Applying cryo electron microscopy to a unique transcription system using 54 (N), the major bacterial variant sigma factor, we capture a new intermediate state at 4.1 Å where promoter DNA is caught at the entrance of the RNAP cleft. Combining with new structures of the open promotercomplex and an initial de novo transcribing complex at 3.4 and 3.7 Å respectively, our studies reveal the dynamics of DNA loading and mechanism of transcription bubble stabilisation that involves coordinated, large scale conformational changes of the universally conserved features within RNAP and DNA. In addition, our studies reveal a novel mechanism of strand separation by 54.
AU  - Glyde,R
AU  - Ye,F
AU  - Jovanovic,M
AU  - Kotta-Loizou,I
AU  - Buck,M
AU  - Zhang,X
DO  - 10.1016/j.molcel.2018.05.021
EP  - 1120
PY  - 2018///
SN  - 1097-2765
SP  - 1111
TI  - Structures of bacterial RNA polymerase complexes reveal mechanisms of DNA loading and transcription initiation
T2  - Molecular Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2018.05.021
UR  - http://hdl.handle.net/10044/1/60236
VL  - 70
ER  - 

Download