@article{Ramlaul:2019:10.1016/j.jsb.2018.11.011,
author = {Ramlaul, K and Palmer, CM and Aylett, CHS},
doi = {10.1016/j.jsb.2018.11.011},
journal = {Journal of Structural Biology},
pages = {30--40},
title = {A local agreement filtering algorithm for transmission EM reconstructions},
url = {http://dx.doi.org/10.1016/j.jsb.2018.11.011},
volume = {205},
year = {2019}
}

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TY  - JOUR
AB  - We present LAFTER, an algorithm for de-noising single particle reconstructions from cryo-EM.Single particle analysis entails the reconstruction of high-resolution volumes from tens of thousands of particle images with low individual signal-to-noise. Imperfections in this process result in substantial variations in the local signal-to-noise ratio within the resulting reconstruction, complicating the interpretation of molecular structure. An effective local de-noising filter could therefore improve interpretability and maximise the amount of useful information obtained from cryo-EM maps.LAFTER is a local de-noising algorithm based on a pair of serial real-space filters. It compares independent half-set reconstructions to identify and retain shared features that have power greater than the noise. It is capable of recovering features across a wide range of signal-to-noise ratios, and we demonstrate recovery of the strongest features at Fourier shell correlation (FSC) values as low as 0.144 over a 2563-voxel cube. A fast and computationally efficient implementation of LAFTER is freely available.We also propose a new way to evaluate the effectiveness of real-space filters for noise suppression, based on the correspondence between two FSC curves: 1) the FSC between the filtered and unfiltered volumes, and 2) Cref, the FSC between the unfiltered volume and a hypothetical noiseless volume, which can readily be estimated from the FSC between two half-set reconstructions.
AU  - Ramlaul,K
AU  - Palmer,CM
AU  - Aylett,CHS
DO  - 10.1016/j.jsb.2018.11.011
EP  - 40
PY  - 2019///
SN  - 1047-8477
SP  - 30
TI  - A local agreement filtering algorithm for transmission EM reconstructions
T2  - Journal of Structural Biology
UR  - http://dx.doi.org/10.1016/j.jsb.2018.11.011
UR  - https://www.sciencedirect.com/science/article/pii/S1047847718303113?via%3Dihub
UR  - http://hdl.handle.net/10044/1/64858
VL  - 205
ER  - 

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