Transgenesis is a complex and fast growing technology. Having been involved in transgenesis and knock out technology for the past 10 years, my aim is to provide expertise for your models, from the gene to experimental analysis.

Issues that I can help you with:

Experimental design for Transgenesis

  • How to clone cDNA or genomic DNA
  • Homologous recombination or random integration
  • Choosing ES cells or pronuclear injection
  • What promoter to drive expression of transgene
  • Constitutive or inducible expression
  • The Cre-Lox system may be suitable
  • Cell-type-specific or ubiquitous
  • How to backcross, genotype and maintain a colony
  • A database of already available transgenic and KO mice - the “mouse locator”