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  • Journal article
    VIPOND IB, BALDWIN GS, HALFORD SE, 1995,

    DIVALENT METAL-IONS AT THE ACTIVE-SITES OF THE ECORV AND ECORI RESTRICTION ENDONUCLEASES

    , BIOCHEMISTRY, Vol: 34, Pages: 697-704, ISSN: 0006-2960
  • Journal article
    BALDWIN GS, VIPOND IB, HALFORD SE, 1995,

    RAPID REACTION ANALYSIS OF THE CATALYTIC CYCLE OF THE ECORV RESTRICTION-ENDONUCLEASE

    , BIOCHEMISTRY, Vol: 34, Pages: 705-714, ISSN: 0006-2960
  • Journal article
    TONEY MD, HOHENESTER E, KELLER JW, JANSONIUS JNet al., 1995,

    STRUCTURAL AND MECHANISTIC ANALYSIS OF 2 REFINED CRYSTAL-STRUCTURES OF THE PYRIDOXAL PHOSPHATE-DEPENDENT ENZYME DIALKYLGLYCINE DECARBOXYLASE

    , JOURNAL OF MOLECULAR BIOLOGY, Vol: 245, Pages: 151-179, ISSN: 0022-2836
  • Conference paper
    DEMAGISTRIS MT, DITOMMASO A, PIZZA M, RAPPUOLI R, DOUGAN G, DOUCE Get al., 1995,

    ADJUVANT EFFECT OF NONTOXIC MUTANTS OF LT FOLLOWING INTRANASAL AND INTRAVAGINAL IMMUNIZATION

    , Publisher: WILEY-LISS, Pages: 245-245, ISSN: 0730-2312
  • Conference paper
    BARCHFELD GL, HESSLER AL, HIGGINS DA, CATALDO D, TRAQUINA P, PIZZA M, DEMAGISTRIS MT, DOUCE G, DOUGAN G, VANNEST Get al., 1995,

    INTRANASAL VACCINATION WITH SUBUNIT INFLUENZA VACCINE COMBINED WITH THE ADJUVANTS LTK63 OR MF59

    , Publisher: WILEY-LISS, Pages: 254-254, ISSN: 0730-2312
  • Journal article
    PAROLIS H, STANLEY SMR, DELL A, REASON AJet al., 1995,

    A PARTIAL REDUCTIVE-CLEAVAGE STUDY OF THE CAPSULAR POLYSACCHARIDE OF ESCHERICHIA-COLI K57

    , CARBOHYDRATE RESEARCH, Vol: 266, Pages: 95-102, ISSN: 0008-6215
  • Journal article
    KING RD, HIRST JD, STERNBERG MJE, 1995,

    COMPARISON OF ARTIFICIAL-INTELLIGENCE METHODS FOR MODELING PHARMACEUTICAL QSARS

    , APPLIED ARTIFICIAL INTELLIGENCE, Vol: 9, Pages: 213-233, ISSN: 0883-9514
  • Journal article
    KEMPF HG, BRANDLE TU, WISDEN W, ZENNER HP, MARX Aet al., 1995,

    GAMMA-AMINOBUTYRIC ACID(A) RECEPTOR, MESSENGERRNA DETECTION IN COCHLEAR SURFACE PREPARATIONS - AN IN-SITU HYBRIDIZATION INVESTIGATION

    , HNO, Vol: 43, Pages: 12-18, ISSN: 0017-6192
  • Journal article
    CUNNINGHAM L, WILLIAMS HD, 1995,

    ISOLATION AND CHARACTERIZATION OF MUTANTS DEFECTIVE IN THE CYANIDE-INSENSITIVE RESPIRATORY PATHWAY OF PSEUDOMONAS-AERUGINOSA

    , JOURNAL OF BACTERIOLOGY, Vol: 177, Pages: 432-438, ISSN: 0021-9193
  • Journal article
    BOBBIONIHARSCH E, FRUTIGER S, HUGHES G, PANICO M, ETIENNE A, ZAPPACOSTA F, MORRIS HR, JEANRENAUD Bet al., 1995,

    PHYSIOLOGICAL CONCENTRATIONS OF OXYTOCIN POWERFULLY STIMULATE INSULIN-SECRETION IN-VITRO

    , ENDOCRINE, Vol: 3, Pages: 55-59, ISSN: 0969-711X
  • Journal article
    CHARALAMBOUS M, SHELLEY AJ, GRACIO AJD, RAYBOULD JNet al., 1995,

    CYTOGENETICAL ANALYSIS OF THE SIMULIUM-DAMNOSUM COMPLEX (DIPTERA, SIMULIIDAE) IN GUINEA-BISSAU

    , MEDICAL AND VETERINARY ENTOMOLOGY, Vol: 9, Pages: 34-42, ISSN: 0269-283X
  • Journal article
    Kempf HG, Brändle TU, Wisden W, Zenner HP, Marx Aet al., 1995,

    [Detection of GABA(A) receptor mRNA in cochlear tissue. An in situ hybridization study].

    , HNO, Vol: 43, Pages: 12-18, ISSN: 0017-6192

    Gamma-aminobutyric acid (GABA) and the GABAergic system play an important role in the efferent modulation of cochlear function. We examined surface preparations of guinea pig and mouse cochleae by in situ hybridization using radioactive labelled oligonucleotides for several subunits of the GABAA receptor. Frozen sections of rat and guinea pig brain (cortex, hippocampus, cerebellum) served as controls. In the mouse cochlea the mRNA of the alpha-1 and alpha-5, beta-1 and gamma-1 subunit were detected, while in guinea pig cochlea mRNA of the alpha-1, alpha-4, alpha-5, and gamma-1 subunit of the GABAA receptor were found. Positive signals were located in the regions of the outer hair cells and had a weaker intensity in the inner hair cells. In the brain sections the several subunits were detected in a variable distribution in the cerebellum, hippocampus and cortical regions. Rat specimens exhibited stronger signals than guinea pig brain sections. These investigations have extended previous results of immunocytochemical experiments from our laboratory demonstrating mRNA sequences of GABAA receptor subunits in the mammalian inner ear. Detection of these nucleotide sequences using surface preparations of the cochlea on a molecular level by in situ hybridization supports the importance of GABA as a cochlear neurotransmitter. Furthermore, it can be concluded that the mammalian cochlea is able to express a GABA-dependent neurotransmission system.
  • Book chapter
    Richardson WD, Pringle NP, Yu W-P, Collarini EJ, Hall ACet al., 1995,

    Embryonic origin and early development of oligodendrocytes.

    , Glial Cell Development, Editors: Jessen, Richardson, Oxford, Publisher: Bios Scientific Publishers, Pages: 53-70
  • Conference paper
    DAVIS DM, BIRCH DJS, GELLERT PR, KITTLETY RS, SWART RMet al., 1995,

    A fluorescence study of Nile red bound to Human Serum Albumin in buffer, denaturant, and reverse micelles

    , 2nd Biannual Conference on Advances in Fluorescence Sensing Technology, Publisher: SPIE - INT SOC OPTICAL ENGINEERING, Pages: 302-313
  • Journal article
    Sternberg MJ, Hegyi H, Islam SA, Luo J, Russell RBet al., 1995,

    Towards an intelligent system for the automatic assignment of domains in globular proteins.

    , Proc Int Conf Intell Syst Mol Biol, Vol: 3, Pages: 376-383, ISSN: 1553-0833

    The automatic identification of protein domains from coordinates is the first step in the classification of protein folds and hence is required for databases to guide structure prediction. Most algorithms encode a single concept based and sometimes do not yield assignments that are consistent with the generally accepted perception. Our development of an automatic approach to identify reliably domains from protein coordinates is described. The algorithm is benchmarked against a manual identification of the domains in 284 representative protein chains. The first step is the domain assignment by distance (DAD) algorithm that considers the density of inter-residue contacts represented in a contact matrix. The algorithm yields 85% agreement with the manual assignment. The paper then considers how the reliability of these assignments could be evaluated. Finally the use of structural comparisons using the STAMP algorithm to validate domain assignment is reported on a test case.
  • Journal article
    Lloyd J, Wong SC, Styles JM, Batten D, Priddle R, Turnbull C, Mcconchie CAet al., 1995,

    Measuring and Modelling Whole-Tree Gas Exchange

    , Australian Journal of Plant Physiology, Vol: 22, Pages: 987-1000

    <P>Diurnal patterns of CO<sub>2</sub> and water vapour exchange were determined for <I>Macadamia integrifolia</I> and <I>Litchi chinensis</I> trees enclosed in whole-tree gas exchange chambers at Alstonville, New South Wales (28.5ºS) during October and November 1991. Whole-tree gas exchange responses to photon irradiance (I), ambient partial pressure of CO<sub>2</sub> (Ca) and vapour pressure deficit (D) were similar to those normally observed for individual leaves. Nevertheless, at a given I (above approximately 500 &#956;mol quanta m<sup>-2</sup>, s<sup>-1</sup>) stomatal conductances (g<sub>s</sub>) and CO<sub>2</sub> assimilation rates (A) were higher under overcast, as opposed to clear sky conditions. This difference was maintained even when A and g<sub>s</sub> were examined as a function of sun angle. Combined with a simple light interception model, nested quadratic equations combining stomatal responses and biochemical characteristics of individual leaves were found to provide excellent descriptions of the gas exchange responses of the isolated trees. This indicates a close to optimal partitioning of photosynthetic machinery throughout the canopy. From the whole-tree gas exchange model it was found that higher A and g<sub>s</sub> under overcast conditions are attributable to both lower T<sub>1</sub> and D as well as to a more uniform distribution of irradiance across the canopy surface.
  • Journal article
    Kok R, van Thor JJ, Nugteren-Roodzant I, Vosman B, Hellingwerf KJet al., 1995,

    Characterization of lipase-deficient mutants of Acinetobacter calcoaceticus BD413: identification of a periplasmic lipase chaperone essential for the production of extracellular lipase

    , J Bact, Pages: 177-3295
  • Journal article
    Kok R, van Thor JJ, Nugteren-Roodzant I, Brouwer MB, Egmond MR, Nudel CB, Vosman B, Hellingwerf KJet al., 1995,

    Characterization of the extracellular lipase, LipA, of Acinetobacter calcoaceticus BD413 and sequence analysis of the cloned structural gene

    , Mol Microbiol, Vol: 15, Pages: 803-818
  • Journal article
    Wisden W, 1995,

    Structure and distribution of multiple GABA-A receptor subunits with special reference to the cerebellum

    , Ann N Y Acad Sci, Vol: 757, Pages: 506-515, ISSN: 0077-8923
  • Book chapter
    Hunt SP, McNaughton LA, Jenkins R, Wisden Wet al., 1995,

    Immediate-early gene activation as a window on mechanism in the nervous system.

    , Immediate-Early Genes in the Central Nervous System, Editors: Tolle, Schadrack, Ziegelgaensberger, Berlin, Publisher: Springer-Verlag, Pages: 18-34
  • Journal article
    Tolle TR, Berthele A, Zieglgansberger W, Seeburg PH, Wisden Wet al., 1995,

    Flip and Flop variants of AMPA receptors in the rat lumbar spinal cord

    , Eur J Neurosci, Vol: 7, Pages: 1414-1419, ISSN: 0953-816X

    The expression of eight messenger RNA splice forms encoding the Flip and Flop variants of AMPA receptor subunits GluR-A to -D in the rat lumbar spinal cord was examined by in situ hybridization using specific oligonucleotides. In the dorsal horn (laminae I, II and III) the predominant mRNA was GluR-B Flip. Much lower levels of GluR-A Flip were found in lamina I and in superficial parts of lamina II outer. In the ventral horn, motor neurons expressed mainly GluR-B Flip, GluR-C Flip and Flop, and GluR-D Flip. Serial sectioning through large motor neurons indicated that a given cell contained, for example, both GluR-C Flip and Flop splice types.
  • Conference paper
    Seidler A, Michel H, Rutherford AW, 1995,

    The extrinsic 33 KDA protein of Photosystem II: Improved expression plasmids and progress in the mutational analysis

    , Xth International Photosynthesis Congress, Publisher: SPRINGER, Pages: 259-262
  • Journal article
    Vipond IB, Baldwin GS, Halford SE, 1995,

    Divalent metal ions at the active sites of the EcoRV and EcoRI restriction endonucleases

    , Biochemistry, Vol: 34, Pages: 705-714
  • Journal article
    Peppoloni S, Pizza M, DeMagistris MT, Bartoloni A, Rappuoli Ret al., 1995,

    Acellular pertussis vaccine composed of genetically inactivated pertussis toxin

    , PHYSIOLOGICAL CHEMISTRY AND PHYSICS AND MEDICAL NMR, Vol: 27, Pages: 355-361, ISSN: 0748-6642
  • Conference paper
    DeMagistris MT, DiTommaso A, Pizza M, Rappuoli Ret al., 1995,

    Rational design of vaccine molecules to prevent pertussis

    , NATO Advanced Study Institute on Vaccines - New Generation Immunological Adjuvants, Publisher: PLENUM PRESS DIV PLENUM PUBLISHING CORP, Pages: 97-102, ISSN: 0258-1213
  • Book chapter
    Melillo JM, Prentice IC, Farquhar GD, Schulze E-D, Sala OEet al., 1995,

    Terrestrial Biotic Responses to Environmental Change and Feedbacks to Climate

    , CLIMATE CHANGE 1995: THE SCIENCE OF CLIMATE CHANGE, Editors: Houghton, Filho, Callander, Harris, Kattenberg, Maskell, Publisher: CAMBRIDGE UNIV PRESS, Pages: 445-481, ISBN: 0-521-56436-0
  • Conference paper
    Geffen A, Nash RDM, 1995,

    Periodicity of otolith check formation in the juvenile plaice Pleuronectes platessa L

    , 1st International Symposium on Fish Otoliths - Research and Application, Publisher: UNIV SOUTH CAROLINA PRESS, Pages: 65-73
  • Conference paper
    Geffen A, 1995,

    Estimation of fish growth - Session II - Overview I

    , 1st International Symposium on Fish Otoliths - Research and Application, Publisher: UNIV SOUTH CAROLINA PRESS, Pages: 77-78
  • Journal article
    SAVOLAINEN V, CUENOUD P, SPICHIGER R, MARTINEZ MDP, CREVECOEUR M, MANEN JFet al., 1995,

    THE USE OF HERBARIUM SPECIMENS IN DNA PHYLOGENETICS - EVALUATION AND IMPROVEMENT

    , PLANT SYSTEMATICS AND EVOLUTION, Vol: 197, Pages: 87-98, ISSN: 0378-2697
  • Conference paper
    Andronis C, Durrant JR, Diner BA, Merry S, Klug DR, Nixon PJet al., 1995,

    Construction and initial characterisation of a D2-Leu205Tyr mutant of Chlamydomonas reinhardtii

    , Xth International Photosynthesis Congress, Publisher: KLUWER ACADEMIC PUBL, Pages: 839-842

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