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• Journal article
  Dickinson R, Lieb WR, Franks NP, 1996,

  The effects of temperature on the interactions between volatile general anaesthetics and a neuronal nicotinic acetylcholine receptor (vol 116, pg 2949, 1995)

  , BRITISH JOURNAL OF PHARMACOLOGY, Vol: 117, Pages: 1369-1369, ISSN: 0007-1188
  • Author Web Link
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  • Citations: 1
• Journal article
  DiTommaso A, Saletti G, Pizza M, Rappuoli R, Dougan G, Abrignani S, Douce G, DeMagistris Met al., 1996,

  Induction of antigen-specific antibodies in vaginal secretions by using a nontoxic mutant of Hsai-Labile enterotoxin as a mucosal adjuvant

  , INFECTION AND IMMUNITY, Vol: 64, Pages: 974-979, ISSN: 0019-9567
  • Author Web Link
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  • Citations: 180
• Journal article
  Clark GF, Oehninger S, Patankar MS, Koistinen R, Dell A, Morris HR, Koistinen H, Seppala Met al., 1996,

  A role for glycoconjugates in human development: The human feto-embryonic defence system hypothesis

  , HUMAN REPRODUCTION, Vol: 11, Pages: 467-473, ISSN: 0268-1161
  • Cite
  • Citations: 105
• Journal article
  vanVliet P, Rutherford AW, 1996,

  Properties of the chloride-depleted oxygen-evolving complex of photosystem II studied by electron paramagnetic resonance

  , BIOCHEMISTRY, Vol: 35, Pages: 1829-1839, ISSN: 0006-2960
  • Author Web Link
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  • Citations: 76
• Journal article
  Drickamer K, 1996,

  Ca2+-dependent sugar recognition by animal lectins

  , BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 24, Pages: 146-150, ISSN: 0300-5127
  • Author Web Link
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  • Citations: 38
• Journal article
  Denesyuk AI, Matthews S, Zavyalov VP, Korpela Tet al., 1996,

  Conservative hydrophobic interdomain contacts of IFN-gamma remain in p17 matrix protein of HIV-1

  , APMIS, Vol: 104, Pages: 141-146, ISSN: 0903-4641
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  • Citations: 1
• Journal article
  Legge JT, Roush R, Desalle R, Vogler AP, May Bet al., 1996,

  Genetic criteria for establishing evolutionarily significant units in Cryan's buckmoth

  , CONSERVATION BIOLOGY, Vol: 10, Pages: 85-98, ISSN: 0888-8892
  • Author Web Link
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  • Citations: 51
• Journal article
  Russell RB, Sternberg MJE, 1996,

  A novel binding site in catalase is suggested by structural similarity to the calycin superfamily

  , PROTEIN ENGINEERING, Vol: 9, Pages: 107-111, ISSN: 0269-2139
  • Author Web Link
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  • Citations: 8
• Journal article
  Crockford AJ, Behncke C, Williams HD, 1996,

  The adaptation of Rhizobium leguminosarum bv phaseoli to oxidative stress and its overlap with other environmental stress responses

  , MICROBIOLOGY-SGM, Vol: 142, Pages: 331-336, ISSN: 1350-0872
  • Author Web Link
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  • Citations: 13
• Journal article
  Prentice IC, Guiot J, Huntley B, Jolly D, Cheddadi Ret al., 1996,

  Reconstructing biomes from palaeoecological data: A general method and its application to European pollen data at 0 and 6 ka

  , CLIMATE DYNAMICS, Vol: 12, Pages: 185-194, ISSN: 0930-7575
  • Cite
  • Citations: 587
• Journal article
  Burrows L, Knight R, Polokoff E, Schanzer H, Panico M, Solomon Met al., 1996,

  Expanding the donor pool with the use of en bloc pediatric kidneys in adult recipients

  , TRANSPLANTATION PROCEEDINGS, Vol: 28, Pages: 173-174, ISSN: 0041-1345
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  • Citations: 19
• Journal article
  Giorgi LB, Nixon PJ, Merry SA, Joseph DM, Durrant JR, De Las Rivas J, Barber J, Porter G, Klug DRet al., 1996,

  Comparison of primary charge separation in the photosystem II reaction center complex isolated from wild-type and D1-130 mutants of the cyanobacterium Synechocystis PCC 6803.

  , J Biol Chem, Vol: 271, Pages: 2093-2101, ISSN: 0021-9258

  We compare primary charge separation in a photosystem II reaction center preparation isolated from a wild-type (WT) control strain of the cyanobacterium Synechocystis sp. PCC 6803 and from two site-directed mutants of Synechocystis in which residue 130 of the D1 polypeptide has been changed from a glutamine to either a glutamate (mutant D1-Gln130Glu), as in higher plant sequences, or a leucine residue (mutant D1-Gln130Leu). The D1-130 residue is thought to be close to the pheophytin electron acceptor. We show that, when P680 is photoselectively excited, the primary radical pair state P680+Ph- is formed with a time constant of 20-30 ps in the WT and both mutants; this time constant is very similar to that observed in Pisum sativum (a higher plant). We also show that a change in the residue at position D1-130 causes a shift in the peak of the pheophytin Qx-band. Nanosecond and picosecond transient absorption measurements indicate that the quantum yield of radical pair formation (phi RP), associated with the 20-30-ps component, is affected by the identify of the D1-130 residue. We find that, for the isolated photosystem II reaction center particle, phi RP higher plant > phi RP D1-Gln130Glu mutant > phi RP WT > phi RP D1-Gln130Leu mutant. Furthermore, the spectroscopic and quantum yield differences we observe between the WT Synechocystis and higher plant photosystem II, seem to be reversed by mutating the D1-130 ligand so that it is the same as in higher plants. This result is consistent with the previously observed natural regulation of quantum yield in Synechococcus PS II by particular changes in the D1 polypeptide amino acid sequence (Clark, A.K., Hurry, V. M., Gustafsson, P. and Oquist, G. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 11985-11989).

  • Abstract
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• Journal article
  Burt A, Carter DA, Koenig GL, White TJ, Taylor JWet al., 1996,

  Molecular markers reveal cryptic sex in the human pathogen Coccidioides immitis

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 93, Pages: 770-773, ISSN: 0027-8424
  • Cite
  • Citations: 392
• Journal article
  Skavdis G, SidenKiamos I, Muller HM, Crisanti A, Louis Cet al., 1996,

  Conserved function of Anopheles gambiae midgut-specific promoters in the fruitfly

  , EMBO JOURNAL, Vol: 15, Pages: 344-350, ISSN: 0261-4189
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  • Citations: 41
• Journal article
  Woodley P, Buck M, Kennedy C, 1996,

  Identification of sequences important for recognition of vnf genes by the VnfA transcriptional activator in Azotobacter vinelandii

  , FEMS MICROBIOLOGY LETTERS, Vol: 135, Pages: 213-221, ISSN: 0378-1097
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  • Citations: 18
• Journal article
  Ng KKS, Drickamer K, Weis WI, 1996,

  Structural analysis of monosaccharide recognition by rat liver mannose-binding protein

  , JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 271, Pages: 663-674, ISSN: 0021-9258
  • Author Web Link
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  • Citations: 151
• Journal article
  King RD, Muggleton SH, Srinivasan A, Sternberg MJEet al., 1996,

  Structure-activity relationships derived by machine learning: The use of atoms and their bond connectivities to predict mutagenicity by inductive logic programming

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 93, Pages: 438-442, ISSN: 0027-8424
  • Cite
  • Citations: 170
• Journal article
  Seppälä M, Oehninge S, Dell A, Morris HR, Koistinen H, Koistinen R, Clark GFet al., 1996,

  The role of glycodelins in regulation of fertilization and implantation: The fertilization window

  , Gynecological Endocrinology, Vol: 10, Pages: 129-131, ISSN: 0951-3590
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• Journal article
  Ray A, Williams HD, 1996,

  A mutant of Pseudomonas aeruginosa that lacks c-type cytochromes has a functional cyanide-insensitive oxidase

  , FEMS MICROBIOLOGY LETTERS, Vol: 135, Pages: 123-129, ISSN: 0378-1097
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  • Citations: 9
• Journal article
  Dickinson R, Lieb WR, Franks NP, 1996,

  Erratum: The effects of temperature on the interactions between volatile general anaesthetics and a neuronal nicotinic acetylcholine receptor (British Journal of Pharmacology (1995) 116 (2949-2956))

  , British Journal of Pharmacology, Vol: 117, ISSN: 0007-1188
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• Patent
  Tjian R, Comai L, Ruppert S, Dynlacht BD, Hoey T, Tanese N, Wang E, Weinzierl ROet al., 1996,

  TATA-binding protein associated factors drug screens

  , 5534410
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• Journal article
  Hohenester E, Maurer P, Hohenadl C, Timpl R, Jansonius JN, Engel Jet al., 1996,

  Structure of a novel extracellular Ca2+-binding module in BM-40

  , NATURE STRUCTURAL BIOLOGY, Vol: 3, Pages: 67-73, ISSN: 1072-8368
  • Author Web Link
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  • Citations: 125
• Journal article
  Rappuoli R, Pizza M, 1996,

  Novel molecular biology approaches to acellular vaccines.

  , Biotechnol Annu Rev, Vol: 2, Pages: 391-408, ISSN: 1387-2656

  Bacterial toxins are commonly detoxified by chemical treatment in order to use them in human vaccines. We have used site-directed mutagenesis of toxin genes to obtain bacteria that produce naturally nontoxic mutants of bacterial toxins, such as pertussis toxin (PT), cholera toxin (CT) and Escherichia coli heat-labile enterotoxin (LT). Genetically detoxified PT showed a superior safety and immunogenicity in animal models, phase I and phase II clinical trials, and a superior protective efficacy in the early and late stage of a phase III efficacy trial, proving in a definitive and extensive way that genetic detoxification of bacterial toxins can, and should, replace chemical treatment. The results obtained with genetically inactivated LT and CT indicate that genetic detoxification of bacterial toxins can be used not only to produce vaccines for systemic immunization that are superior to the ones produced by conventional technologies, but suggest that these type of molecules may be the prototype molecules for the design and construction of innovative vaccines with a totally new design, such as mucosally delivered preventive and therapeutic vaccines.

  • Abstract
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• Conference paper
  Giorgi LB, Nixon PJ, Merry SAP, Joseph DM, Durrant JR, De las Rivas J, Barber J, Porter G, Klug DRet al., 1996,

  Comparison of PS II primary photochemistry in higher plant, synechocystis and synechocystis mutants

  , Current Research in Photosynthesis, Publisher: Kluwer Academic Publishers
  • Cite
• Journal article
  Rappuoli R, Pizza M, Douce G, Dougan Get al., 1996,

  New vaccines against bacterial toxins.

  , Adv Exp Med Biol, Vol: 397, Pages: 55-60, ISSN: 0065-2598
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• Conference paper
  Shen BW, Hennig M, Hohenester E, Jansonius JNet al., 1996,

  THREE DIMENSIONAL STRUCTURE OF HUMAN ORNITHINE AMINOTRANSFERASE: CHARGE DISTRIBUTION AT THE ACTIVE SITE EXPLAINS THE SELECTIVITY FOR W - GROUP OF DIBASIC AMINO ACIDS.

  , Publisher: INT UNION CRYSTALLOGRAPHY, Pages: C113-C113, ISSN: 2053-2733
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• Conference paper
  Burkhard P, Hohenester E, Rao GSJ, Cook PF, Jansonius JNet al., 1996,

  THREE-DIMENSIONAL STRUCTURE OF O-ACETYLSERINE SULFHYDRYLASE FROM SALMONELLA TYPHIMURIUM

  , Publisher: INT UNION CRYSTALLOGRAPHY, Pages: C125-C126, ISSN: 2053-2733
  • Author Web Link
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  • Citations: 1
• Journal article
  Turnbull CGN, Anderson KL, Winston EC, 1996,

  Influence of gibberellin treatment on flowering and fruiting patterns in mango

  , AUSTRALIAN JOURNAL OF EXPERIMENTAL AGRICULTURE, Vol: 36, Pages: 603-611, ISSN: 0816-1089
  • Author Web Link
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  • Citations: 17
• Book
  Sternberg MJE, 1996,

  Protein Structure Prediction - A Practical Approach

  , Oxford, Publisher: Oxford University Press
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• Journal article
  Morris HR, Paxton T, Dell A, Langhorne J, Berg M, Bordoli RS, Hoyes J, Bateman RHet al., 1996,

  High sensitivity collisionally-activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer

  , RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Vol: 10, Pages: 889-896, ISSN: 0951-4198
  • Author Web Link
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  • Citations: 364

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