@article{Pennington:2018:10.1101/291427,
author = {Pennington, HG and Jones, R and Kwon, S and Bonciani, G and Thieron, H and Chandler, T and Luong, P and Morgan, S and Przydacz, M and Bozkurt, T and Bowden, S and Craze, M and Wallington, E and Garnett, J and Kwaaitaal, M and Panstruga, R and Cota, E and Spanu, PD},
doi = {10.1101/291427},
title = {A fungal ribonuclease-like effector protein inhibits plant host ribosomal RNA degradation},
url = {http://dx.doi.org/10.1101/291427},
year = {2018}
}

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TY  - JOUR
AB  - <jats:title>ABSTRACT</jats:title><jats:p>The biotrophic fungal pathogen<jats:italic>Blumeria graminis</jats:italic>causes the powdery mildew disease of cereals and grasses. Proteins with a predicted ribonuclease (RNase)-like fold (termed RALPHs) comprise the largest set of secreted effector candidates within the<jats:italic>B. graminis</jats:italic>f. sp.<jats:italic>hordei</jats:italic>genome. Their exceptional abundance suggests they play crucial functions during pathogenesis. We show that transgenic expression of RALPH CSEP0064/BEC1054 increases susceptibility to infection in monocotyledenous and dicotyledonous plants. CSEP0064/BEC1054 interacts<jats:italic>in planta</jats:italic>with five host proteins: two translation elongation factors (eEF1α and eEF1γ), two pathogenesis-related proteins (PR5 and PR10) and a glutathione-S-transferase. We present the first crystal structure of a RALPH, CSEP0064/BEC1054, demonstrating it has an RNase-like fold. The protein interacts with total RNA and weakly with DNA. Methyl jasmonate levels modulate susceptibility to aniline-induced host RNA fragmentation.<jats:italic>In planta</jats:italic>expression of CSEP0064/BEC1054 reduces the formation of this RNA fragment. We propose that CSEP0064/BEC1054 is a pseudoenzyme that binds to host ribosomes, thereby inhibiting the action of plant ribosome-inactivating proteins that would otherwise lead to host cell death, an unviable interaction and demise of the fungus.</jats:p>
AU  - Pennington,HG
AU  - Jones,R
AU  - Kwon,S
AU  - Bonciani,G
AU  - Thieron,H
AU  - Chandler,T
AU  - Luong,P
AU  - Morgan,S
AU  - Przydacz,M
AU  - Bozkurt,T
AU  - Bowden,S
AU  - Craze,M
AU  - Wallington,E
AU  - Garnett,J
AU  - Kwaaitaal,M
AU  - Panstruga,R
AU  - Cota,E
AU  - Spanu,PD
DO  - 10.1101/291427
PY  - 2018///
TI  - A fungal ribonuclease-like effector protein inhibits plant host ribosomal RNA degradation
UR  - http://dx.doi.org/10.1101/291427
ER  - 

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