@article{Katsanos:2022:10.1126/sciadv.abk3141,
author = {Katsanos, D and Barkoulas, M},
doi = {10.1126/sciadv.abk3141},
journal = {Science Advances},
pages = {1--15},
title = {Targeted DamID in C. elegans reveals a direct role for LIN-22 and NHR-25 in antagonising the epidermal stem cell fate},
url = {http://dx.doi.org/10.1126/sciadv.abk3141},
volume = {8},
year = {2022}
}

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TY  - JOUR
AB  - Transcription factors are key players in gene networks controlling cell fate specification during development. In multicellular organisms, they often display complex patterns of expression  and  binding  to  their  targets,  hence  tissue-specificity  is  required  in  the characterisation of transcription factor-target interactions. We introduce here Targeted DamID (TaDa) as a method for tissue-specific transcription factor target identification in intact C. elegans animals.  We employ TaDa to recover targets in the epidermis for two key transcription factors, the HES1 homologue LIN-22 and the NR5A1/2 nuclear hormone receptor NHR-25. We demonstrate a direct link between LIN-22 and the Wnt signalling pathway through repression of the Frizzled receptor lin-17. We also find a direct role for NHR-25  in  promoting  cell  differentiation  via  repressing  the  expression  of  stem  cell-promoting GATA factors. Our results expand our understanding of the epidermal gene network and highlight the potential of TaDa to dissect the architecture of tissue-specific gene regulatory networks.
AU  - Katsanos,D
AU  - Barkoulas,M
DO  - 10.1126/sciadv.abk3141
EP  - 15
PY  - 2022///
SN  - 2375-2548
SP  - 1
TI  - Targeted DamID in C. elegans reveals a direct role for LIN-22 and NHR-25 in antagonising the epidermal stem cell fate
T2  - Science Advances
UR  - http://dx.doi.org/10.1126/sciadv.abk3141
UR  - https://www.science.org/doi/10.1126/sciadv.abk3141
UR  - http://hdl.handle.net/10044/1/93253
VL  - 8
ER  - 

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