@article{Miracca:2022:10.1523/JNEUROSCI.0350-21.2022,
author = {Miracca, G and Anuncibay, Soto B and Tossell, K and Yustos, R and Vyssotski, A and Franks, N and Wisden, W},
doi = {10.1523/JNEUROSCI.0350-21.2022},
journal = {The Journal of Neuroscience},
pages = {5389--5409},
title = {NMDA receptors in the lateral preoptic hypothalamus are essential for sustaining NREM and REM sleep},
url = {http://dx.doi.org/10.1523/JNEUROSCI.0350-21.2022},
volume = {42},
year = {2022}
}

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TY  - JOUR
AB  - The lateral preoptic (LPO) hypothalamus is a center for NREM and REM sleep induction and NREM sleep homeostasis. Although LPO is needed for NREM sleep, we found that calcium signals were, surprisingly, highest in REM sleep. Furthermore, and equally surprising, NMDA26 receptors in LPO were the main drivers of excitation. Deleting the NMDA receptor GluN1 subunit from LPO abolished calcium signals in all cells and produced insomnia. Mice of both sexes had highly fragmented NREM sleep-wake patterns and could not generate conventionally classified REM sleep. The sleep phenotype produced by deleting NMDA receptors depended on where in the hypothalamus the receptors were deleted. Deleting receptors from the anterior hypothalamic area did not influence sleep-wake states. The sleep fragmentation originated from NMDA receptors on GABA neurons in LPO. Sleep fragmentation could be transiently overcome with sleeping medication (zolpidem) or sedatives (dexmedetomidine). By contrast, fragmentation persisted under high sleeppressure produced by sleep deprivation - mice had a high propensity to sleep but woke up. By analyzing changes in delta power, sleep homeostasis (also referred to as “sleep drive”) remained intact after NMDA receptor ablation. We suggest NMDA glutamate receptor activation stabilizes firing of sleep-on neurons, and that mechanisms of sleep maintenance differ from that of the sleep drive itself.
AU  - Miracca,G
AU  - Anuncibay,Soto B
AU  - Tossell,K
AU  - Yustos,R
AU  - Vyssotski,A
AU  - Franks,N
AU  - Wisden,W
DO  - 10.1523/JNEUROSCI.0350-21.2022
EP  - 5409
PY  - 2022///
SN  - 0270-6474
SP  - 5389
TI  - NMDA receptors in the lateral preoptic hypothalamus are essential for sustaining NREM and REM sleep
T2  - The Journal of Neuroscience
UR  - http://dx.doi.org/10.1523/JNEUROSCI.0350-21.2022
UR  - https://www.jneurosci.org/content/42/27/5389
UR  - http://hdl.handle.net/10044/1/96701
VL  - 42
ER  - 

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