Citation

BibTex format

@article{McClure:2022:10.7554/eLife.67598,
author = {McClure, C and Aughey, G and Hassan, A and Butt, K and Estacio, Gomez A and Duggal, A and Ying, Sia C and Barber, A and Southall, T},
doi = {10.7554/eLife.67598},
journal = {eLife},
pages = {1--18},
title = {An auxin-inducible, GAL4-compatible, gene expression system for Drosophila},
url = {http://dx.doi.org/10.7554/eLife.67598},
volume = {11},
year = {2022}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The ability to control transgene expression, both spatially and temporally, is essential for studying model organisms. In Drosophila, spatial control is primarily provided by the GAL4/UAS system, whilst temporal control relies on a temperature-sensitive GAL80 (which inhibits GAL4) and drug-inducible systems. However, these are not ideal. Shifting temperature can impact on many physiological and behavioural traits, and the current drug-inducible systems are either leaky, toxic, incompatible with existing GAL4-driver lines, or do not generate effective levels of expression. Here, we describe the auxin-inducible gene expression system (AGES). AGES relies on the auxin-dependent degradation of a ubiquitously expressed GAL80, and therefore, is compatible with existing GAL4-driver lines. Water-soluble auxin is added to fly food at a low, non-lethal, concentration, which induces expression comparable to uninhibited GAL4 expression. The system works in both larvae and adults, providing a stringent, non-lethal, cost-effective, and convenient method for temporally controlling GAL4 activity in Drosophila.
AU - McClure,C
AU - Aughey,G
AU - Hassan,A
AU - Butt,K
AU - Estacio,Gomez A
AU - Duggal,A
AU - Ying,Sia C
AU - Barber,A
AU - Southall,T
DO - 10.7554/eLife.67598
EP - 18
PY - 2022///
SN - 2050-084X
SP - 1
TI - An auxin-inducible, GAL4-compatible, gene expression system for Drosophila
T2 - eLife
UR - http://dx.doi.org/10.7554/eLife.67598
UR - https://elifesciences.org/articles/67598
UR - http://hdl.handle.net/10044/1/96127
VL - 11
ER -