@article{Fu:2022:10.1128/jvi.00318-22,
author = {Fu, M and Zhang, H and Yin, M and Han, Z and Bai, Q and Peng, Y and Shafik, K and Zhai, L and Hong, N and Xu, W and Wang, G and Kotta-Loizou, I},
doi = {10.1128/jvi.00318-22},
journal = {Journal of Virology},
pages = {1--14},
title = {A novel heptasegmented positive-sense single-stranded RNA virus from the phytopathogenic fungus colletotrichum fructicola},
url = {http://dx.doi.org/10.1128/jvi.00318-22},
volume = {96},
year = {2022}
}

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TY  - JOUR
AB  - In this study, a novel positive-sense single-stranded RNA (+ssRNA) mycovirus, tentatively named Colletotrichum fructicola RNA virus 1 (CfRV1), was identified in the phytopathogenic fungus Colletotrichum fructicola. CfRV1 has seven genomic components, encoding seven proteins from open reading frames (ORFs) flanked by highly conserved untranslated regions (UTRs). Proteins encoded by ORFs 1, 2, 3, 5, and 6 are more similar to the putative RNA-dependent RNA polymerase (RdRp), hypothetical protein (P2), methyltransferase, and two hypothetical proteins of Hadaka virus 1 (HadV1), a capsidless 10- or 11-segmented +ssRNA virus, while proteins encoded by ORFs 4 and 7 showed no detectable similarity to any known proteins. Notably, proteins encoded by ORFs 1 to 3 also share considerably high similarity with the corresponding proteins of polymycoviruses. Phylogenetic analysis conducted based on the amino acid sequence of CfRV1 RdRp and related viruses placed CfRV1 and HadV1 together in the same clade, close to polymycoviruses and astroviruses. CfRV1-infected C. fructicola strains demonstrate a moderately attenuated growth rate and virulence compared to uninfected isolates. CfRV1 is capsidless and potentially encapsulated in vesicles inside fungal cells, as revealed by transmission electron microscopy. CfRV1 and HadV1 are +ssRNA mycoviruses closely related to polymycoviruses and astroviruses, represent a new linkage between +ssRNA viruses and the intermediate double-stranded RNA (dsRNA) polymycoviruses, and expand our understanding of virus diversity, taxonomy, evolution, and biological traits. IMPORTANCE A scenario proposing that dsRNA viruses evolved from +ssRNA viruses is still considered controversial due to intergroup knowledge gaps in virus diversity. Recently, polymycoviruses and hadakaviruses were found as intermediate dsRNA and +ssRNA stages, respectively, between +ssRNA and dsRNA viruses. Here, we identified a novel +ssRNA mycovirus, Colletotrichum fructicola RNA virus
AU  - Fu,M
AU  - Zhang,H
AU  - Yin,M
AU  - Han,Z
AU  - Bai,Q
AU  - Peng,Y
AU  - Shafik,K
AU  - Zhai,L
AU  - Hong,N
AU  - Xu,W
AU  - Wang,G
AU  - Kotta-Loizou,I
DO  - 10.1128/jvi.00318-22
EP  - 14
PY  - 2022///
SN  - 0022-538X
SP  - 1
TI  - A novel heptasegmented positive-sense single-stranded RNA virus from the phytopathogenic fungus colletotrichum fructicola
T2  - Journal of Virology
UR  - http://dx.doi.org/10.1128/jvi.00318-22
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35435725
UR  - https://journals.asm.org/doi/10.1128/jvi.00318-22
UR  - http://hdl.handle.net/10044/1/96690
VL  - 96
ER  - 

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