Citation

BibTex format

@article{D'Amato:2024:10.1038/s41467-024-44907-x,
author = {D'Amato, R and Taxiarchi, C and Galardini, M and Trusso, A and Minuz, RL and Grilli, S and Somerville, AGT and Shittu, D and Khalil, AS and Galizi, R and Crisanti, A and Simoni, A and Müller, R},
doi = {10.1038/s41467-024-44907-x},
journal = {Nat Commun},
title = {Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages.},
url = {http://dx.doi.org/10.1038/s41467-024-44907-x},
volume = {15},
year = {2024}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - CRISPR-based gene drives have the potential to spread within populations and are considered as promising vector control tools. A doublesex-targeting gene drive was able to suppress laboratory Anopheles mosquito populations in small and large cages, and it is considered for field application. Challenges related to the field-use of gene drives and the evolving regulatory framework suggest that systems able to modulate or revert the action of gene drives, could be part of post-release risk-mitigation plans. In this study, we challenge an AcrIIA4-based anti-drive to inhibit gene drive spread in age-structured Anopheles gambiae population under complex feeding and behavioural conditions. A stochastic model predicts the experimentally-observed genotype dynamics in age-structured populations in medium-sized cages and highlights the necessity of large-sized cage trials. These experiments and experimental-modelling framework demonstrate the effectiveness of the anti-drive in different scenarios, providing further corroboration for its use in controlling the spread of gene drive in Anopheles.
AU - D'Amato,R
AU - Taxiarchi,C
AU - Galardini,M
AU - Trusso,A
AU - Minuz,RL
AU - Grilli,S
AU - Somerville,AGT
AU - Shittu,D
AU - Khalil,AS
AU - Galizi,R
AU - Crisanti,A
AU - Simoni,A
AU - Müller,R
DO - 10.1038/s41467-024-44907-x
PY - 2024///
TI - Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages.
T2 - Nat Commun
UR - http://dx.doi.org/10.1038/s41467-024-44907-x
UR - https://www.ncbi.nlm.nih.gov/pubmed/38296981
VL - 15
ER -