In this section
@article{Piper:1980:10.1002/9780470720615.ch11,
author = {Piper, PJ and Samhoun, MN and Tippins, JR and Morris, HR and Taylor, GW},
doi = {10.1002/9780470720615.ch11},
journal = {Ciba Found Symp},
pages = {203--215},
title = {Slow-reacting substances and their structural elucidation.},
url = {http://dx.doi.org/10.1002/9780470720615.ch11},
volume = {78},
year = {1980}
}
TY - JOUR
AB - For more than forty years since their discovery, the structure of a group of closely related materials known collectively as slow-reacting substances has been unknown. These substances are released from a variety of tissues in response to immunological or non-immunological stimulation. A slow-reacting substance is believed to be implicated in hypersensitivity reactions such as asthma; in order to fully understand its bronchoconstrictor role, the structural elucidation of these materials has been a necessary (albeit difficult) task. Studies on both immunologically generated slow-reacting substance of anaphylaxis (SRS-A) and other slow-reacting substances (SRSs) have indicated a precursor role for arachidonic acid in their biosynthesis; this, coupled with enzymic and chemical activity destruction data, gave an insight into the structure of these moieties. In order to define the structure of these materials homogeneous SRS-A was required; a purification scheme was developed relying on the high resolution separative capability of reverse-phase high pressure liquid chromatography, resulting in extensively purified SRS-A. It was then possible to demonstrate that SRS-A possessed a characteristic ultraviolet spectrum, allowing us for the first time to define a major structural moiety in the molecule (conjugated triene). To complement studies on, and to act as a model for the more pathologically relevant SRS-A, a slow-reacting substance was produced from rat basophilic leukaemia (RBL-1) cells. The structure of this biologically active species has been determined by mass spectrometric examination of the intact molecule as a derivative, together with analytical protein chemical studies, and shown to be the novel peptidolipid 5-hydroxy-6-cysteinylglycinyl-7,9,11,14-eicosatetraenoic acid.
AU - Piper,PJ
AU - Samhoun,MN
AU - Tippins,JR
AU - Morris,HR
AU - Taylor,GW
DO - 10.1002/9780470720615.ch11
EP - 215
PY - 1980///
SN - 0300-5208
SP - 203
TI - Slow-reacting substances and their structural elucidation.
T2 - Ciba Found Symp
UR - http://dx.doi.org/10.1002/9780470720615.ch11
UR - https://www.ncbi.nlm.nih.gov/pubmed/6110523
VL - 78
ER -
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