@article{Aydemir:2018:infdis/jiy223,
author = {Aydemir, O and Janko, M and Hathaway, NJ and Verity, R and Mwandagalirwa, MK and Tshefu, AK and Tessema, SK and Marsh, PW and Tran, A and Reimonn, T and Ghani, AC and Ghansah, A and Juliano, JJ and Greenhouse, BR and Emch, M and Meshnick, SR and Bailey, JA},
doi = {infdis/jiy223},
journal = {Journal of Infectious Diseases},
pages = {946--955},
title = {Drug-resistance and population structure of plasmodium falciparum across the Democratic Republic of Congo using high-throughput molecular inversion probes},
url = {http://dx.doi.org/10.1093/infdis/jiy223},
volume = {218},
year = {2018}
}

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TY  - JOUR
AB  - A better understanding of the drivers of the spread of malaria parasites and drug resistance across space and time is needed. These drivers can be elucidated using genetic tools. Here, a novel molecular inversion probe (MIP) panel targeting all major drug-resistance mutations and a set of microsatellites was used to genotype Plasmodium falciparum infections of 552 children from the 2013–2014 Demographic and Health Survey conducted in the Democratic Republic of the Congo (DRC). Microsatellite-based analysis of population structure suggests that parasites within the DRC form a homogeneous population. In contrast, sulfadoxine-resistance markers in dihydropteroate synthase show marked spatial structure with ongoing spread of double and triple mutants compared with 2007. These findings suggest that parasites in the DRC remain panmictic despite rapidly spreading antimalarial-resistance mutations. Moreover, highly multiplexed targeted sequencing using MIPs emerges as a cost-effective method for elucidating pathogen genetics in complex infections in large cohorts.
AU  - Aydemir,O
AU  - Janko,M
AU  - Hathaway,NJ
AU  - Verity,R
AU  - Mwandagalirwa,MK
AU  - Tshefu,AK
AU  - Tessema,SK
AU  - Marsh,PW
AU  - Tran,A
AU  - Reimonn,T
AU  - Ghani,AC
AU  - Ghansah,A
AU  - Juliano,JJ
AU  - Greenhouse,BR
AU  - Emch,M
AU  - Meshnick,SR
AU  - Bailey,JA
DO  - infdis/jiy223
EP  - 955
PY  - 2018///
SN  - 0022-1899
SP  - 946
TI  - Drug-resistance and population structure of plasmodium falciparum across the Democratic Republic of Congo using high-throughput molecular inversion probes
T2  - Journal of Infectious Diseases
UR  - http://dx.doi.org/10.1093/infdis/jiy223
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000441792600013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/jid/article/218/6/946/4989954
UR  - http://hdl.handle.net/10044/1/75829
VL  - 218
ER  - 

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