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  • Journal article
    Percy MG, Gruendling A, 2014,

    Lipoteichoic Acid Synthesis and Function in Gram-Positive Bacteria

    , ANNUAL REVIEW OF MICROBIOLOGY, VOL 68, Vol: 68, Pages: 81-100, ISSN: 0066-4227
  • Journal article
    Pean CB, Dionne MS, 2014,

    Intracellular infections in Drosophila melanogaster: Host defense and mechanisms of pathogenesis

  • Journal article
    Clark RI, Walker DW, Dionne MS, 2014,

    Metabolic and immune integration in aging and age-related disease

    , AGING-US, Vol: 6, Pages: 3-4, ISSN: 1945-4589
  • Journal article
    Liu B, Shadrin A, Sheppard C, Mekler V, Xu Y, Severinov K, Matthews S, Wigneshweraraj Set al., 2014,

    The sabotage of the bacterial transcription machinery by a small bacteriophage protein.

    , Bacteriophage, Vol: 4, ISSN: 2159-7073

    Many bacteriophages produce small proteins that specifically interfere with the bacterial host transcription machinery and thus contribute to the acquisition of the bacterial cell by the bacteriophage. We recently described how a small protein, called P7, produced by the Xp10 bacteriophage inhibits bacterial transcription initiation by causing the dissociation of the promoter specificity sigma factor subunit from the host RNA polymerase holoenzyme. In this addendum to the original publication, we present the highlights of that research.

  • Journal article
    Jones C, Filloux A, 2014,

    Gene amplification and qRT-PCR.

    , Methods Mol Biol, Vol: 1149, Pages: 457-468

    This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell lysate from Pseudomonas species for colony PCR reactions. The chapter continues with a switch in focus from DNA to RNA, describing methods for RNA isolation from Pseudomonas, cDNA generation, and finally q-RT-PCR to investigate relative changes in gene expression.

  • Journal article
    Pader V, James EH, Painter KL, Wigneshweraraj S, Edwards AMet al., 2014,

    The Agr quorum-sensing system regulates fibronectin-binding but not hemolysis in the absence of a functional electron transport chain.

    , Infection and Immunity
  • Journal article
    Filloux A, Ramos J-L, 2014,

    Preface. Pseudomonas methods and protocols.

    , Methods Mol Biol, Vol: 1149
  • Journal article
    Muhl D, Filloux A, 2014,

    Site-directed mutagenesis and gene deletion using reverse genetics.

    , Methods Mol Biol, Vol: 1149, Pages: 521-539

    Understanding gene function is far easier when tools are available to engineer a bacterial strain lacking a specific gene and phenotypically compare its behavior with the corresponding parental strain. Such mutants could be selected randomly, either by natural selection under particular stress conditions or by random mutagenesis using transposon delivery as described elsewhere in this book. However, with the advent of the genomic era there are now hundreds of bacterial genomes whose sequence is available, and thus, genes can be identified, chosen, and strategies designed to specifically inactivate them. This can be done by using suicide plasmids and is most convenient when the bacterium of interest is easily amenable to genetic manipulation. The method presented here will describe the use of a suicide vector, pKNG101, which allows the selection of a double-recombination event. The first event results in the integration of the pKNG101 derivative carrying the "mutator" fragment onto the chromosome, and could be selected on plates containing appropriate antibiotics. The pKNG101 carries the sacB gene, which induces death when cells are grown on sucrose. Growth on sucrose plates will thus select the second homologous recombination event, which results in removing the plasmid backbone and leaving behind the mutated target gene. This method has been widely used over the last 20 years to inactivate genes in a wide range of gram-negative bacteria and in particular in Pseudomonas aeruginosa.

  • Journal article
    Barraud N, Moscoso JA, Ghigo J-M, Filloux Aet al., 2014,

    Methods for studying biofilm dispersal in Pseudomonas aeruginosa.

    , Methods Mol Biol, Vol: 1149, Pages: 643-651

    Biofilm dispersal is the last and least understood stage of the biofilm life cycle. Several recent studies have characterized dispersal events in response to various cues and signals. Here we describe a range of methods useful for the investigation of dispersal in the biofilm model organism and opportunistic pathogen Pseudomonas aeruginosa.

  • Journal article
    Filloux A, 2013,

    Fit and resistant is a worst case scenario with bacterial pathogens


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