@article{Pavlidis:2019:10.1183/13993003.00938-2018,
author = {Pavlidis, S and Takahashi, K and Kwong, FNK and Xie, J and Hoda, U and Sun, K and Elyasigomari, V and Agapow, P and Loza, M and Baribaud, F and Chanez, P and Fowler, SJ and Shaw, DE and Fleming, LJ and Howarth, PH and Sousa, AR and Corfield, J and Auffray, C and De, Meulder B and Knowles, R and Sterk, PJ and Guo, Y and Adcock, IM and Djukanovic, R and Chung, KF and U-BIOPRED, study group},
doi = {10.1183/13993003.00938-2018},
journal = {European Respiratory Journal},
title = {"T2-high" in severe asthma related to blood eosinophil, exhaled nitric oxide and serum periostin},
url = {http://dx.doi.org/10.1183/13993003.00938-2018},
volume = {53},
year = {2019}
}

Download

TY  - JOUR
AB  - BACKGROUND: Type-2 (T2) immune responses in airway epithelial cells (AECs) classifies mild-moderate asthma into a T2-high phenotype. We examined whether currently-available clinical biomarkers can predict AEC-defined T2-high phenotype within U-BIOPRED cohort. METHODS: The transcriptomic profile of AECs obtained from brushings of 103 patients with asthma and 44 healthy controls was obtained and gene set variation analysis used to determine the relative expression score of T2 asthma using a signature from IL-13-exposed AECs. RESULTS: 37% of asthmatics (45% non-smoking severe asthma, n=49, 33% of smoking or ex-smoking severe asthma, n=18 and 28% mild-moderate asthma, n=36) were T2-high using AEC gene expression. They were more symptomatic with higher levels of nitric oxide in exhaled breath (FeNO) and of blood and sputum eosinophils but not of serum IgE or periostin. Sputum eosinophilia correlated best with the T2-high signature. FeNO (≥30ppb) and blood eosinophils (≥300/µL) gave a moderate prediction of T2-high asthma. Sputum IL-4, IL-5 and IL-13 protein levels did not correlate with gene expression. CONCLUSION: T2-high severe asthma can be predicted to some extent from raised levels of FeNO, blood and sputum eosinophil counts, but serum IgE or serum periostin were poor predictors. Better bedside biomarkers are needed to detect T2-high.
AU  - Pavlidis,S
AU  - Takahashi,K
AU  - Kwong,FNK
AU  - Xie,J
AU  - Hoda,U
AU  - Sun,K
AU  - Elyasigomari,V
AU  - Agapow,P
AU  - Loza,M
AU  - Baribaud,F
AU  - Chanez,P
AU  - Fowler,SJ
AU  - Shaw,DE
AU  - Fleming,LJ
AU  - Howarth,PH
AU  - Sousa,AR
AU  - Corfield,J
AU  - Auffray,C
AU  - De,Meulder B
AU  - Knowles,R
AU  - Sterk,PJ
AU  - Guo,Y
AU  - Adcock,IM
AU  - Djukanovic,R
AU  - Chung,KF
AU  - U-BIOPRED,study group
DO  - 10.1183/13993003.00938-2018
PY  - 2019///
SN  - 0903-1936
TI  - "T2-high" in severe asthma related to blood eosinophil, exhaled nitric oxide and serum periostin
T2  - European Respiratory Journal
UR  - http://dx.doi.org/10.1183/13993003.00938-2018
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30578390
UR  - http://hdl.handle.net/10044/1/65419
VL  - 53
ER  - 

Download