@article{Erriah:2019:10.1186/s12931-018-0967-9,
author = {Erriah, M and Pabreja, K and Fricker, M and Baines, KJ and Donnelly, LE and Bylund, J and Karlsson, A and Simpson, JL},
doi = {10.1186/s12931-018-0967-9},
journal = {Respiratory Research},
title = {Galectin-3 enhances monocyte-derived macrophage efferocytosis of apoptotic granulocytes in asthma},
url = {http://dx.doi.org/10.1186/s12931-018-0967-9},
volume = {20},
year = {2019}
}

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TY  - JOUR
AB  - BackgroundGalectin-3 is a 32 kDa protein secreted by macrophages involved in processes such as cell activation, chemotaxis and phagocytosis. Galectin-3 has previously been shown to improve the ability of airway macrophages to ingest apoptotic cells (efferocytosis) in chronic obstructive pulmonary disease (COPD) and may be of interest in non-eosinophilic asthma (NEA) which is also characterised by impaired efferocytosis. It was hypothesised that the addition of exogenous galectin-3 to monocyte-derived macrophages (MDMs) derived from donors with NEA would enhance their ability to engulf apoptotic granulocytes.MethodsEligible non-smoking adults with asthma (n = 19), including 7 with NEA and healthy controls (n = 10) underwent a clinical assessment, venepuncture and sputum induction. MDMs were co-cultured with apoptotic granulocytes isolated from healthy donors with or without exogenous recombinant galectin-3 (50 μg/mL) and efferocytosis was assessed by flow cytometry. Galectin-3 expression and localisation in MDMs was visualised by immunofluorescence staining and fluorescence microscopy. Galectin-3, interleukin (IL)-6 and CXCL8 secretion were measured in cell culture supernatants by ELISA and cytometric bead array.ResultsBaseline efferocytosis (mean (±standard deviation)) was lower in participants with asthma (33.2 (±17.7)%) compared with healthy controls (45.3 (±15.9)%; p = 0.081). Efferocytosis did not differ between the participants with eosinophilic asthma (EA) (31.4 (±19.2)%) and NEA (28.7 (±21.5)%; p = 0.748). Addition of galectin-3 significantly improved efferocytosis in asthma, particularly in NEA (37.8 (±18.1)%) compared with baseline (30.4 (±19.7)%; p = 0.012). Efferocytosis was not associated with any of the clinical outcomes but was negatively correlated with sputum macrophage numbers (Spearman r = −&thi
AU  - Erriah,M
AU  - Pabreja,K
AU  - Fricker,M
AU  - Baines,KJ
AU  - Donnelly,LE
AU  - Bylund,J
AU  - Karlsson,A
AU  - Simpson,JL
DO  - 10.1186/s12931-018-0967-9
PY  - 2019///
SN  - 1465-9921
TI  - Galectin-3 enhances monocyte-derived macrophage efferocytosis of apoptotic granulocytes in asthma
T2  - Respiratory Research
UR  - http://dx.doi.org/10.1186/s12931-018-0967-9
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000454899500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/66778
VL  - 20
ER  - 

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