@article{Bardin:2018:10.1038/s41598-018-28665-7,
author = {Bardin, EE and Cameron, SJS and Perdones-Montero, A and Hardiman, K and Bolt, F and Alton, EWFW and Bush, A and Davies, JC and Takats, Z},
doi = {10.1038/s41598-018-28665-7},
journal = {Scientific Reports},
title = {Metabolic phenotyping and strain characterisation of pseudomonas aeruginosa Isolates from cystic fibrosis patients using rapid evaporative ionisation mass spectrometry},
url = {http://dx.doi.org/10.1038/s41598-018-28665-7},
volume = {8},
year = {2018}
}

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TY  - JOUR
AB  - Rapid evaporative ionisation mass spectrometry (REIMS) is a novel technique for the real-time analysis of biological material. It works by conducting an electrical current through a sample, causing it to rapidly heat and evaporate, with the analyte containing vapour channelled to a mass spectrometer. It was used to characterise the metabolome of 45 Pseudomonas aeruginosa (P. aeruginosa) isolates from cystic fibrosis (CF) patients and compared to 80 non-CF P. aeruginosa. Phospholipids gave the highest signal intensity; 17 rhamnolipids and 18 quorum sensing molecules were detected, demonstrating that REIMS has potential for the study of virulence-related metabolites. P. aeruginosa isolates obtained from respiratory samples showed a higher diversity, which was attributed to the chronic nature of most respiratory infections. The analytical sensitivity of REIMS allowed the detection of a metabolome that could be used to classify individual P. aeruginosa isolates after repeated culturing with 81% accuracy, and an average 83% concordance with multilocus sequence typing. This study underpins the capacities of REIMS as a tool with clinical applications, such as metabolic phenotyping of the important CF pathogen P. aeruginosa, and highlights the potential of metabolic fingerprinting for fine scale characterisation at a sub-species level.
AU  - Bardin,EE
AU  - Cameron,SJS
AU  - Perdones-Montero,A
AU  - Hardiman,K
AU  - Bolt,F
AU  - Alton,EWFW
AU  - Bush,A
AU  - Davies,JC
AU  - Takats,Z
DO  - 10.1038/s41598-018-28665-7
PY  - 2018///
SN  - 2045-2322
TI  - Metabolic phenotyping and strain characterisation of pseudomonas aeruginosa Isolates from cystic fibrosis patients using rapid evaporative ionisation mass spectrometry
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-018-28665-7
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000439102400008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/61707
VL  - 8
ER  - 

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